DIET AND GENOTYPE IN PRIMATE ATHEROSCLEROSIS: PHENOTYPING CORE

灵长类动脉粥样硬化的饮食和基因型：表型核心

• 批准号: 8172667
• 负责人: DAVID L RAINWATER
• 金额: $ 11.62万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172667
• 关键词: Aliquot; Antioxidants; Apolipoprotein E; Apolipoproteins B; Arterial Fatty Streak; Arteries; Atherogenic Diet; Atherosclerosis; Autopsy; Biochemical; Biological Assay; Biological Markers; Blood; Blood specimen; C-reactive protein; Cardiovascular Diseases; Clinical Chemistry; Computer Retrieval of Information on Scientific Projects Database; Culture Media; Data; Diet; E-Selectin; Functional disorder; Funding; Gel; Genotype; Grant; High Density Lipoprotein Cholesterol; Institution; Intercellular adhesion molecule 1; Laboratories; Lipoproteins; Methodology; Methods; Papio; Phenotype; Plasma; Primates; Process; Property; Proteins; Protocols documentation; Research; Research Personnel; Resources; Risk Factors; Sampling; Source; Triglycerides; United States National Institutes of Health; Vascular Cell Adhesion Molecule-1; aryldialkylphosphatase; base; enzyme activity; feeding; human NOS3 protein; lipoprotein-associated phospholipase A(2); macrophage; meetings; oxidized low density lipoprotein; programs; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Phenotyping Core Laboratory (Core Unit A) will serve all projects on a highly interactive basis. Core Unit A has seven objectives. Objective 1 is to acquire, process, aliquot, and store blood samples as they come into the laboratory. On average, 426 serum and plasma samples/year will come in for processing. Objective 2 is to manage these and all other program-related samples currently in storage. Objective 3 is to quantify indicators of endothelial dysfunction in blood samples and culture medium. There will be an average of 213 blood samples/year and 326 samples/year of culture medium, in which as many as six endothelial functional markers will be assessed. The six biomarkers are E-selectin, endothelial nitric oxide synthase, and macrophage chemotactic protein 1, which will be assayed only in culture medium, and vascular cell adhesion molecule 1, intercellular adhesion molecule 1, and von Willibrand's factor, which will be assayed in both blood and culture medium samples. Objective 4 is to quantify, in an average of 139 samples per year, a panel of established risk factors for cardiovascular disease in blood samples from baboons undergoing an experiment that involves feeding an atherogenic diet for two years before a necropsy protocol for assessing extent of atherosclerotic lesions. The risk factors to be quantified include several analytes assayed using standard clinical chemistry methods (total and HDL cholesterol, triglyceride, apoAI, apoB, apoE, total antioxidant status, and C-reactive protein) and several specialized biochemical assays of established risk factors (lipoprotein size properties using gradient gel electrophoretic methods, enzyme activities of paraoxonase and lipoprotein-associated phospholipase A2, and concentrations of oxidized LDL). Objective 5 is to quantify extent of atherosclerotic lesions in artery samples taken at necropsy. Objective 6 is to establish new methodologies as required. Finally, Objective 7 is to collect and validate the data produced in this Core Unit and to make them available for analysis by Program investigators. The activities proposed for Core Unit A will provide a rich resource of samples and data that will be required to meet the Aims of the Projects in this Program.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 表型核心实验室（核心单元）将以高度互动的基础为所有项目提供服务。核心单元A有七个目标。目标1是在进入实验室时获取，处理，等分试样和存储血液样本。平均而言，将有426个血清和血浆样品/年来处理。 目标2是管理当前正在存储的这些与程序相关的所有其他样本。目标3是量化血液样本和培养基中内皮功能障碍的指标。平均将有213个血液样本和326个样本/年培养基，其中将评估多达6个内皮功能标记。六种生物标志物是E-选择蛋白，内皮一氧化氮合酶和巨噬细胞趋化蛋白1，仅在培养基中进行测定，以及血管细胞粘附分子1，细胞间粘附分子1和Von Willibrand的因素，将在血液和培养基中分析。目标4是要在每年平均139个样本中量化一批已建立的危险因素，从狒狒的血液样本中，经过一项实验，涉及将动脉粥样硬化饮食喂养两年，然后在评估动脉粥样硬化病变程度的尸检方案之前喂食动脉粥样硬化饮食。要量化的风险因素包括使用标准临床化学方法（总和HDL胆固醇，甘油三酸酯，ApoAI，Apob，ApoB，ApoE，APOE，总抗氧化剂状态和C反应蛋白）和几种专业化的生物化学危险因素（使用Lipopropoprototion size Propieres pareagon pareagon pareagon pare careax geel metletoss careax gere props，脂蛋白相关磷脂酶A2和浓度 氧化的LDL）。目标5是为了量化在尸检时进行的动脉样品中动脉粥样硬化病变的程度。目标6是根据需要建立新的方法。最后，目标7是收集和验证该核心单元中产生的数据，并使计划调查人员可以分析它们。 核心单位A提出的活动将提供丰富的样本和数据资源，以满足该计划中项目的目标。