WNPRC STEM CELL RESOURCE

WNPRC干细胞资源

• 批准号: 8173102
• 负责人: James Alexander Thomson
• 金额: $ 10.33万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173102
• 关键词: Adult; Callithrix; Cell Line; Cells; Communication; Communities; Computer Retrieval of Information on Scientific Projects Database; Culture Techniques; Derivation procedure; Disease; ES Cell Line; Embryo; Fibroblast Growth Factor 2; Fibroblasts; Freezing; Funding; Future; Generations; Grant; Human; Institution; Journals; Knowledge; Macaca mulatta; Methods; Paper; Peer Review; Plasmids; Pluripotent Stem Cells; Primates; Proteins; Protocols documentation; Publications; Recombinant Proteins; Reporting; Research; Research Institute; Research Personnel; Resource Allocation; Resources; Science; Services; Source; Stem Cell Research; Stem cells; Time; Training; Transgenes; United States National Institutes of Health; Wisconsin; Work; Zebrafish; base; embryonic stem cell; induced pluripotent stem cell; interest; investigator training; journal article; meetings; member; plasmid DNA; research study; vector; Adult; Callithrix; Cell Line; Cells; Communication; Communities; Computer Retrieval of Information on Scientific Projects Database; Culture Techniques; Derivation procedure; Disease; ES Cell Line; Embryo; Fibroblast Growth Factor 2; Fibroblasts; Freezing; Funding; Future; Generations; Grant; Human; Institution; Journals; Knowledge; Macaca mulatta; Methods; Paper; Peer Review; Plasmids; Pluripotent Stem Cells; Primates; Proteins; Protocols documentation; Publications; Recombinant Proteins; Reporting; Research; Research Institute; Research Personnel; Resource Allocation; Resources; Science; Services; Source; Stem Cell Research; Stem cells; Time; Training; Transgenes; United States National Institutes of Health; Wisconsin; Work; Zebrafish; base; embryonic stem cell; induced pluripotent stem cell; interest; investigator training; journal article; meetings; member; plasmid DNA; research study; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To maintain and further develop a specialized resource for studies relating to pluripotent stem cell research. Allocation of Resource Access: To date, the stem cell resource unit at the Wisconsin National Primate Research Center provides frozen rhesus and marmoset ES cells to interested investigators. No request has been denied. Additionally, the stem cell resource unit provides zebrafish bFGF for culturing primate ES cells. Since last submission 3 new investigators have received the recombinant protein bringing the total number of investigators receiving zbFGF on a regular basis to 21. Over 330mg of zbFGF has been distributed to date. This has saved investigators over $1,000,000. The DNA plasmid used to purify the protein itself is now available through Addgene (www.addgene.com) and was sent to 13 new investigators in 2008 bringing the total number of investigators to receive this plasmid to 39. Lastly, in 2009, 2 investigators requested and received rhesus ES cell provided by the stem cell resources unit and distributed by the WiCell Research Institute. Dissemination: Knowledge is disseminated to the scientific community via publications in peer reviewed journals and scientific meeting attendance. The Wisconsin National Primate Research Center also holds quarterly research retreats to create increased communication between the various service and resource units. Training: Training in culture techniques of primate embryonic stem cells is available. Many new investigators have taken advantage of this resource in previous reporting periods however there have been no new investigators trained this year. Progress: Past and present members of stem cell resources developed a method for generating iPS cells without vector transgene sequences; this work is referenced below in the highlights portion. We have provided ips cell derivation for disease specific cell lines for several UW investigators. To date, 4 disease cell lines have been used for ips cell generation and a total of 55 clones have been cultured and frozen for future use. We are also beginning to start reprogramming experiments on rhesus macaque fibroblasts. Other groups have successfully reprogrammed primate cells and we will be following similar protocols. Highlights: Past and present members of stem cell resources were authors on a paper detailing a vector free method of adult cell reprogramming: Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Junying Yu, Kejin Hu, Kim Smuga-Otto, Shulan Tian, Ron Stewart, Igor I. Slukvin, James A. Thomson Science 8 May 2009 Vol 324. No. 5928, pp 797-801. Challenges: Due to funding shortages we were unable to receive cynomologous embryos and the project came to a halt. We look forward to working with CPI to receive Mauritian cynomolougous embryos to create new cynomologous ES cell lines as described in our P51 proposal. Concerns: No concerns at this time. Stem Cell Resource support is involved in numerous journal articles that depend in part or in full on WNPRC resources.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 目的：维护并进一步开发了与多能干细胞研究有关的研究专门资源。 资源访问的分配： 迄今为止，威斯康星州国家灵长类动物研究中心的干细胞资源单元向有兴趣的研究人员提供了冷冻的恒河类和摩尔莫斯ES细胞。没有拒绝要求。此外，干细胞资源单元提供斑马鱼BFGF用于培养灵长类动物ES细胞。自上次提交以来，3名新调查人员已收到重组蛋白，将ZBFGF的调查人员定期提高到21。迄今已分发了超过330毫克的ZBFGF。这为调查人员节省了超过1,000,000美元。现在可以通过Addgene（www.addgene.com）获得用于纯化蛋白质本身的DNA质粒，并于2008年被送往13位新的研究人员，将研究人员总数接收到39个。最后，2009年，2009年，2名调查人员要求并收到了由干细胞资源单位提供的Rhesus ES细胞，并由Wicell Research Institute分发了。 传播： 知识通过同行评审期刊和科学会议出勤的出版物传播给科学界。威斯康星州国家灵长类动物研究中心还举行季度研究务虚会，以在各种服务和资源单位之间提高沟通。 训练： 可以接受灵长类动物胚胎干细胞的培养技术培训。许多新的调查人员在以前的报告期间利用了这一资源，但是今年没有接受过新的调查人员培训。 进步： 干细胞资源的过去和现在成员开发了一种生成没有载体转基因序列的IPS细胞的方法。这项工作在下面的重点部分中引用。我们为几个UW研究者提供了针对疾病特定细胞系的IPS细胞推导。迄今为止，IPS细胞的产生已使用4种疾病细胞系，总共培养了55个克隆并冷冻以供将来使用。我们也开始在恒河猕猴成纤维细胞上重新编程实验。其他组已成功地重编程了灵长类动物细胞，我们将遵循类似的方案。 亮点： 干细胞资源的过去和现在成员是一篇论文中的作者，详细介绍了一种成年细胞重编程的矢量免费方法：人类诱导的多能干细胞无载体和转基因序列Junying Yu，Kejin Hu，Kim Smuga-Tian，Shulan Tian，Shulan Tian，Ron Stewart，Ron Stewart，Ron Stewart，Ron Stewart，Ron Stewart，Ron Stewart，Ron Stewart，Igor I.Slukvin，I.Slukvin，I.Slukvin，I.Slukvin，James Scicems Science 8 Mays Science 8 32. 59. 2009. 2009. 2009年。 797-801。 挑战： 由于资金短缺，我们无法收到C胚胚，该项目停止了。我们期待与CPI合作，以接收毛里求斯的cynomolougous胚胎，如我们的p51提案中所述，创建新的cynomologologiol ES细胞系。 问题： 目前没有问题。 干细胞资源支持涉及许多期刊文章，部分依赖于WNPRC资源。