SPECIMEN PREP & STAINING TECH FOR MAPPING PROTEINS, CELL COMPLEXES, & PATHWAYS

样本制备

• 批准号: 8169598
• 负责人: GINA E SOSINSKY
• 金额: $ 15.54万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169598
• 关键词: Area; Biological Preservation; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Electron energy loss spectroscopy; Fluorescence; Funding; Grant; Image; Institution; Label; Maps; Metals; Microscopy; Multimodal Imaging; Multiprotein Complexes; Pathway interactions; Plant Resins; Preparation; Proteins; Quantum Dots; Research; Research Personnel; Resources; Sampling; Source; Specimen; Staining method; Stains; Technology; Tissues; United States National Institutes of Health; Work; improved; nanoparticle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. SPECIMEN PREPARATION, PRESERVATION AND STAINING TECHNOLOGIES FOR MAPPING PROTEINS, CELLULAR COMPLEXES, PATHWAYS AND THE SUPRAMOLECULAR ORGANIZATION OF TISSUES USING MULTIPLE LABELS AND MULTISCALE IMAGING. Our efforts in this area are focused on developing technologies to enable simultaneous imaging of proteins and macromolecular complexes using multiple labels and multiscale microscopies. These approaches include (1) new metal chelating derivatives of diaminobenzidine that can be used for simultaneous multi-protein EM labeling using electron energy loss spectroscopy (EELS); (2) enhancing the sensitivity of our repertoire of fluorescent compounds used for multiscale imaging; and (3) improved preservation of probe fluorescence, antigenicity, and cellular ultrastructure in resin-embedded samples to facilitate new imaging applications. In addition, we are working to extend the usefulness of fluorescent nanoparticles (e.g., quantum dots) as probes for multiscale, multimodal imaging.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 使用多种标签和多尺度成像绘制蛋白质、细胞复合物、通路和组织超分子组织图谱的样本制备、保存和染色技术。 我们在这一领域的努力重点是开发技术，利用多个标签和多尺度显微镜对蛋白质和大分子复合物进行同步成像。 这些方法包括（1）二氨基联苯胺的新型金属螯合衍生物，可用于使用电子能量损失光谱（EELS）同时进行多蛋白质电磁标记； (2) 提高我们用于多尺度成像的荧光化合物库的灵敏度； (3) 改进树脂包埋样品中探针荧光、抗原性和细胞超微结构的保存，以促进新的成像应用。此外，我们正在努力扩展荧光纳米颗粒（例如量子点）作为多尺度、多模态成像探针的用途。