ACTIN-MEDIATED CONTRACTILITY EFFECTS ON CAPILLARY MORPHOGENESIS IN TISSUES

肌动蛋白介导的组织毛细血管形态发生的收缩效应

• 批准号: 8170960
• 负责人: Andrew J Putnam
• 金额: $ 4.15万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170960
• 关键词: Actins; Blood capillaries; Computer Retrieval of Information on Scientific Projects Database; Fibrin; Funding; Grant; Hydrogels; Implant; Institution; Mediating; Modeling; Morphogenesis; PECAM1 gene; Research; Research Personnel; Resources; Source; Tissue Stains; Tissues; United States National Institutes of Health; angiogenesis; capillary; inhibitor/antagonist; Actins; Blood capillaries; Computer Retrieval of Information on Scientific Projects Database; Fibrin; Funding; Grant; Hydrogels; Implant; Institution; Mediating; Modeling; Morphogenesis; PECAM1 gene; Research; Research Personnel; Resources; Source; Tissue Stains; Tissues; United States National Institutes of Health; angiogenesis; capillary; inhibitor/antagonist

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A bead-angiogenesis model is implanted in fibrin hydrogels of varied stiffness in this study. They are furthermore treated with actin contractility inhibitors. Angiogenic vessel sprouts that develop on the beads are then analyzed in terms of actin contractility presence as well as morphological differences. The tissues are stained for actin as well as CD31 to determine the effects of contractility inhibition on capillary morphogenesis.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在这项研究中，将珠血管生成模型植入了刚度各异的纤维蛋白水凝胶中。 此外，它们还接受了肌动蛋白收缩力抑制剂的治疗。然后，根据肌动蛋白的收缩力以及形态学差异来分析在珠子上发展的血管生成血管芽。 对肌动蛋白和CD31进行染色，以确定收缩力抑制对毛细血管形态发生的影响。