CHARACTERIZATION OF PKA RIIALPHA-INTERACTING PARTNERS

PKA RIIALPHA 相互作用伙伴的表征

• 批准号: 8171469
• 负责人: ANGEL VELASCO
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171469
• 关键词: Biogenesis; Computer Retrieval of Information on Scientific Projects Database; Cyclic AMP-Dependent Protein Kinases; Data; Dissociation; Enzymes; Event; Funding; Golgi Apparatus; Grant; Institution; Ligands; Maintenance; Mammalian Cell; Membrane; Normal Cell; Organelles; Physiology; Process; Research; Research Personnel; Resources; Role; Route; Shapes; Signal Transduction; Source; Travel; United States National Institutes of Health; reconstruction; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The role of Golgi-associated PKA activity is being investigated in terms of signal transmission and the physiology of the organelle. Recent data indicate that Golgi-associated PKA is basically involved in the maintenance of Golgi structural organization which in the case of mammalian cells corresponds to the typical continuous membranous ribbon shape. Thus, Golgi fragmentation occurs following PKA dissociation or inactivation. Indeed, PKA activity is required to achieve complete Golgi reconstruction during biogenesis. In addition, PKA associated to Golgi membranes becomes activated in the course of a normal cell stimulation process by extracelular ligands. This events triggers important adaptative structural changes in Golgi organization including the fusion of cisternae and miscompartmentalization of processing enzymes. These changes could be indicative of alternative routes followed by cargo molecules travelling across the Golgi stacks.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 通过信号传递和细胞器的生理学，正在研究高尔基体相关PKA活性的作用。最近的数据表明，高尔基体相关的PKA基本上参与了高尔基结构组织的维持，在哺乳动物细胞的情况下，这与典型的连续膜色带形状相对应。因此，在PKA解离或灭活后发生高尔基体碎片。实际上，需要PKA活性以实现生物发生过程中的完全高尔基体重建。 此外，在正常的细胞刺激过程中，与高尔基膜相关的PKA被细胞外配体激活。这一事件触发了高尔基组织的重要适应性结构变化，包括蓄水池的融合和加工酶的分室化。这些变化可能表明替代路线，然后是货物分子穿过高尔基体堆栈。