REGULATION OF CYTOPLASMIC DYNEIN BASED VESICLE TRANSPORT

基于细胞质动力蛋白的囊泡运输的调节

• 批准号: 8171285
• 负责人: TRINA A SCHROER
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171285
• 关键词: Adaptor Signaling Protein; Binding; Cells; Computer Retrieval of Information on Scientific Projects Database; DCTN2 gene; Dynein ATPase; Elements; Funding; Glues; Grant; Institution; Microtubules; Modification; Molecular; Motor; Proteins; Regulation; Research; Research Personnel; Resources; Source; Structure; TNFRSF5 gene; Transport Vesicles; United States National Institutes of Health; Work; base; dynactin; p150(Glued); protein complex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dynactin is a multisubunit protein complex that works in conjunction with the microtubule-based motor, cytoplasmic dynein, to provide a wide range of subcellular localization and motile functions. Dynactin assists dynein by enhancing motor processivity and serving as an adaptor protein that allows dynein to interact with a variety of subcellular cargoes. To do this, dynactin relies on the distinct binding activities of different elements of its structure. The cargo-binding and dynein-binding domains allow dynactin to serve as a dynein adaptor, while processivity enhancement depends on the microtubule-binding and dynein-binding domains. All known cytoplasmic dynein-based transport requires dynactin's structural integrity. The dynactin subunit dynamitin (p50) acts as a molecular "glue" that anchors the dynein and microtubule-binding subunit of dynactin, p150(Glued), to the cargo-binding element. Within dynactin, dynamitin makes contacts with at least three other dynactin subunits as well as itself. This project is focused on obtaining new information on dynactin subunit organization, modification of dynactin subunits, and identifying proteins with which dynactin interacts in cells.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 Dynactin 是一种多亚基蛋白复合物，与基于微管的运动细胞质动力蛋白结合使用，提供广泛的亚细胞定位和运动功能。 Dynactin 通过增强运动持续性并充当接头蛋白来帮助动力蛋白，使动力蛋白与各种亚细胞货物相互作用。 为此，动力蛋白依赖于其结构中不同元素的独特结合活性。 货物结合和动力蛋白结合域允许动力蛋白充当动力蛋白适配器，而持续合成能力的增强取决于微管结合和动力蛋白结合域。所有已知的基于细胞质动力蛋白的运输都需要动力蛋白的结构完整性。 dynactin 亚基 dynamitin (p50) 充当分子“胶水”，将 dynactin 的动力蛋白和微管结合亚基 p150(Glued) 锚定到货物结合元件上。在 dynactin 内，dynamitin 与至少三个其他 dynactin 亚基及其自身接触。 该项目的重点是获得有关 dynactin 亚基组织、dynactin 亚基修饰以及识别细胞中与 dynactin 相互作用的蛋白质的新信息。