IDENTIFICATION OF FAB-7 BOUNDARY PROTEINS

FAB-7 边界蛋白的鉴定

• 批准号: 8171260
• 负责人: Paul D Schedl
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171260
• 关键词: Adult; Binding; Chromatin; Cis-Acting Sequence; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA Sequence; Development; Distal; Drosophila genus; Elements; Embryo; Embryonic Development; Ensure; Eukaryota; Funding; Grant; Institution; Proteins; Research; Research Personnel; Resources; Side; Site; Source; Staging; Tissues; Trans-Activators; United States National Institutes of Health; fly; nuclease; protein complex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Fab-7 boundary functions to ensure the autonomous activity of the iab-6 and iab-7 cis-regulatory domains in the Drosophila Bithorax Complex from early embryogenesis through to the adult stage. Although Fab-7 is only required for the proper development of a single posterior parasegment, it is active in all tissues and stages of development that have been examined. In this respect Fab-7 resembles conventional constitutive boundaries in flies and other eukaryotes that act through ubiquitous cis-elements and trans-acting factors. The Fab-7 boundary spans a DNA sequence of ~1.2 kb and contains 3 chromatin specific nuclease hypersensitive sites. We have examined the functioning of cis-acting sequences in the largest of these hypersensitive sites, HS1, in detail. Though Fab-7 boundary activity is constitutive, we found that HS1 is divided into sub-elements whose boundary activity is restricted to only a specific stage in development. One sub-element, which is located on the proximal side of HS1, is active only in the early embryo. A second sub-element, which is located in the middle of HS1, is not active in early embryos; however, it becomes active mid-way through embryogenesis and then remains active through to the adult stage. A third sub-element on the distal side of HS1 confers boundary activity from early through mid-embryogenesis but not later. Through this project, we identified candidate proteins that are likely to confer this early-stage specific boundary activity, and then found that these proteins make a heterogeneous protein complex to bind the target sequence. We also would like to identify the trans-acting factors that confer other stage specific boundary activity in each.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 FAB-7边界的功能可确保果蝇Bithorax复合物中IAB-6和IAB-7顺式调节结构域的自主活性，从早期胚胎发生到成人阶段。 尽管Fab-7仅对于单个后链球群的正常发育需要，但它在所有组织和发育阶段都活跃。 在这方面，Fab-7类似于通过无处不在的顺式元素和反式作用因素作用的苍蝇和其他真核生物中的常规本构边界。 FAB-7边界跨越约1.2 kb的DNA序列，并包含3个染色质核酸酶超敏位点。 我们已经详细介绍了这些高度敏感位点中最大的S1中的顺式作用序列的功能。 尽管Fab-7边界活动是组成型，但我们发现HS1分为子元素，其边界活动仅限于开发中的特定阶段。 一个位于HS1近端的子元素仅在早期胚胎中活跃。 位于HS1中部的第二个子元素在早期胚胎中不活跃。但是，它在胚胎发生中中途变为活跃，然后一直活跃到成人阶段。 HS1远端的第三个子元素赋予了从早期到中期发生的边界活动，但不以后。 通过该项目，我们确定了可能赋予这种早期特异性边界活动的候选蛋白，然后发现这些蛋白质使异质蛋白复合物具有结合靶序列的异质蛋白。 我们还想确定赋予每种阶段特定边界活动的跨作用因子。