ROLES FOR CASEIN KINASE 2 IN KINETOCHORE REGULATION

酪蛋白激酶 2 在动粒调节中的作用

• 批准号: 8171239
• 负责人: Yuandong Peng
• 金额: $ 0.08万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171239
• 关键词: CCL14 gene; Catalytic Domain; Cell division; Cell physiology; Centromere; Chromosome Segregation; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; Ensure; Eukaryota; Event; Funding; Grant; Homologous Gene; Human; Institution; Kinetochores; Link; Microtubules; Mitotic spindle; Monitor; Phosphotransferases; Proteins; Regulation; Research; Research Personnel; Resources; Role; Saccharomyces cerevisiae; Source; United States National Institutes of Health; Yeasts; casein kinase II; centromere protein C; genome-wide; protein complex; yeast two hybrid system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The kinetochore is a multi-protein complex that assembles on the centromere and attaches chromosomes to spindle microtubules during cell division. Kinetochore attachments are tightly monitored to ensure faithful chromosome segregation. A recent genome-wide yeast two-hybrid screen focusing on the mitotic spindle identified interactions between the essential kinetochore protein Mif2 (S. cerevisiae homolog of human CENP-C) and two Casein Kinase 2 (CK2) subunits, Cka2 and Ckb2. CK2, a ubiquitous and highly conserved kinase in eukaryotes, is composed of two catalytic subunits (Cka1 and Cka2) and two regulatory subunits (Ckb1 and Ckb2). Although CK2 has been linked to a variety of cellular processes, how CK2 regulates these diverse events is largely unclear. In this study, we investigated the roles of CK2 in mitotic spindle function.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 着丝粒是一种多蛋白复合物，在细胞分裂过程中在着丝粒上组装并将染色体附着到纺锤体微管上。着丝粒附着受到严格监控，以确保忠实的染色体分离。最近针对有丝分裂纺锤体的全基因组酵母双杂交筛选发现了必需着丝粒蛋白 Mif2（人类 CENP-C 的酿酒酵母同源物）和两个酪蛋白激酶 2 (CK2) 亚基 Cka2 和 Ckb2 之间的相互作用。 CK2是真核生物中普遍存在且高度保守的激酶，由两个催化亚基（Cka1和Cka2）和两个调节亚基（Ckb1和Ckb2）组成。尽管 CK2 与多种细胞过程有关，但 CK2 如何调节这些不同的事件尚不清楚。在这项研究中，我们研究了 CK2 在有丝分裂纺锤体功能中的作用。