TRAINING IN MASS SPECTROMETRY AND PROTEOMICS -- MACCOSS LAB 2009-2010

质谱和蛋白质组学培训 -- MACCOSS 实验室 2009-2010

• 批准号: 8171317
• 负责人: Michael MacCoss
• 金额: $ 1万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171317
• 关键词: Blood; Blood capillaries; Cell Line; Claw; Code; Computer Retrieval of Information on Scientific Projects Database; Data; Data Analyses; Data Files; Dental Cementum; Digestion; Educational process of instructing; Electronic Mail; Funding; Grant; Hand; High Pressure Liquid Chromatography; Hour; Institutes; Institution; Ions; Isotope Labeling; Mass Spectrum Analysis; Methods; Peptides; Preparation; Proteins; Proteomics; Reader; Research; Research Personnel; Resources; Running; SHFM1 gene; Sampling; Schedule; Site Visit; Source; Testing; Training; United States National Institutes of Health; Universities; Update; Water; capillary; data acquisition; instrument; meetings; operation; research study; software development; sound; symposium; working group

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Training Provided by Brendan MacLean on the Use of Skyline 1. Taught 3 hours of hands-on tutorials for CPTAC Verification Working Group Operators meeting at Broad Institute 2. Created 2 x 25 minute training videos for Skyline v0.5 3. Regular bimonthly instructional updates over WebEx for Verification Working Group 4. Regular WebEx sessions with Susan Abbatiello and Birgit Schilling, leaders of Verification Working Group 5. Multi-day site visit at Vanderbilt University, Liebler and Tabb labs 6. Presentation to Aebersold Lab in Zurich 7. Presentation at U. Zurich mini-symposium 8. Multi-day site visit at Broad Institute, Carr lab 9. Co-taught 1.5 hours of US HUPO quantitative proteomics course 10. Created 3 x 10-20 page tutorials in using Skyline v0.6 11. Answered 110 support requests on Skyline support board (many others through direct email) 12. WebEx sessions with Christina Ludwig of Aebersold Lab 13. Taught undergraduate Alana Killeen professional software development for independent study Training Provided by Jesse Canterbury on General Mass Spectrometry Analysis 1. Rob Moritz, Jeff Stevens (ISB): FAIMS, triple quad operation & data 2. interpretation 3. Matt Chambers (Vanderbilt): generating data files for unit tests of file reader code 4. Shawna Hengel (Med Chem, Goodlett): ECD experiments 5. Scott Shaffer (Med Chem, Goodlett): ion funnel & Velos troubleshooting 6. Jason Wooden (Puget Sound Blood Center): mass spec & HPLC operation 7. Katrina Claw (Swanson lab): mass spec operation 8. Jan Aagaard (Swanson lab): mass spec operation 9. Andrew Stergachis (Stamatoyannopoulos lab): mass spec operation 10. Sean McIlwain (Noble): DIA data interpretation 11. Mirela Andronescu (Noble): data conversion/interpretation Training Provided by Daniela Tomazela on General Mass Spectrometry Analysis 1. Michael Paulsen: (BChE) Skyline, Column Packing, Data Acquisition 2. Francisco Nociti Jr. (Cementum project) Sample Preparation: Tryptic Digestion, Data Acquisition, MSDaPl 3. Ling Wang (Baker Lab): Data Acquisition 4. Judit Marsillach (Furlong lab / OP Project): Tryptic Digestion, MCX Peptide Sample Cleanup, Column Packing 5. Andrew Stergachis (Stamatoyannopoulos lab): Skyline, MS Method setup, SRM data Analysis 6. Jason Wooden (Puget Sound Blood Center): Skyline, LTQ SRM data acquisition, SRM data analysis 7. Tomas Vaisar (Heinecke Lab): Scheduled SRM using Skyline Training Provided by Ed Hsieh on General Mass Spectrometry Analysis 1. Katrina Claw (Swanson Lab): packing capillary columns and use of Agilent 1100 HPLCs 2. Dao-Fu Dao (Rabinovitch Lab): packing capillary columns, with sample analysis on Waters nanoAcquity and Thermo LTQ-FT instruments and with data analysis. 3. Peter Straub (Vanderbilt): in data analysis with Bullseye. 4. Lea Starita (Fields Lab): in data analysis with Bullseye and capillary column packing. Training Provided by Gennifer Merrihew 1. MSDaPl / MacCoss lab pipeline help - Evvie Vincow, Lea Starita, Jan Aagard, Melody Rynerson, Katrina Claw, Renee George, Angela Poole 2. Orbitrap runs, sample prep discussions - Evvie Vincow, Lea Starita 3. GelFree - Lea Starita, Andrew Stergachis, Chuck Witkowski, Jeremy Norris 4. LTQ analysis help, column making help - Melody Rynerson, Katrina Claw, Renee George, Jan Aagard, Angela Poole, Lea Starita 5. Ancient Protein Sample Prep - Ben Novak, Tom Kaye, Matthew Fitzgibbon, Pavel Pezner, Martin McIntosh 6. Isotope labeling discussions - Soledad Undurraga 7. TAP-tagged FOXO cell lines - Matt Kaeberlein, Lara Shamieh

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 Brendan MacLean 提供有关 Skyline 使用的培训 1. 在 Broad Institute 为 CPTAC 验证工作组操作员会议讲授 3 小时的实践教程 2. 为 Skyline v0.5 创建 2 x 25 分钟的培训视频 3. 通过 WebEx 进行验证工作组的定期双月指导更新 4. 与验证工作组负责人 Susan Abbatiello 和 Birgit Schilling 定期举行 WebEx 会议 5. 范德比尔特大学、Liebler 和 Tabb 实验室多日实地考察 6. 向苏黎世 Aebersold 实验室做演讲 7. 在苏黎世大学小型研讨会上的演讲 8. 布罗德研究所卡尔实验室多日实地考察 9. 共同教授美国HUPO定量蛋白质组学课程1.5小时 10. 创建了 3 x 10-20 页的使用 Skyline v0.6 的教程 11. 回答了 Skyline 支持板上的 110 个支持请求（许多其他请求是通过直接电子邮件） 12. 与 Aebersold 实验室的 Christina Ludwig 进行 WebEx 会议 13. 教授本科生Alana Killeen独立学习专业软件开发 Jesse Canterbury 提供一般质谱分析培训 1. Rob Moritz、Jeff Stevens (ISB)：FAIMS、三重四极杆操作和数据 2. 解释 3. Matt Chambers (Vanderbilt)：生成用于文件读取器代码单元测试的数据文件 4. Shawna Hengel（Med Chem，Goodlett）：ECD 实验 5. Scott Shaffer（Med Chem、Goodlett）：离子漏斗和 Velos 故障排除 6. Jason Wooden（普吉特海湾血液中心）：质谱和 HPLC 操作 7. Katrina Claw（斯旺森实验室）：质谱操作 8. Jan Aagaard（斯旺森实验室）：质谱操作 9. Andrew Stergachis（Stamatoyannopoulos 实验室）：质谱操作 10. Sean McIlwain (Noble)：DIA 数据解释 11. Mirela Andronescu (Noble)：数据转换/解释 Daniela Tomazela 提供关于通用质谱分析的培训 1. Michael Paulsen：（BChE）Skyline、装柱、数据采集 2. Francisco Nociti Jr.（水泥项目）样品制备：胰蛋白酶消化、数据采集、MSDaPl 3. Ling Wang（贝克实验室）：数据采集 4. Judit Marsillach（Furlong 实验室/OP 项目）：胰蛋白酶消化、MCX 肽样品净化、装柱 5. Andrew Stergachis（Stamatoyannopoulos 实验室）：Skyline、MS 方法设置、SRM 数据分析 6. Jason Wooden（普吉特海湾血液中心）：Skyline、LTQ SRM数据采集、SRM数据分析 7. Tomas Vaisar（Heinecke 实验室）：使用 Skyline 安排 SRM Ed Hsieh 提供一般质谱分析培训 1. Katrina Claw（Swanson 实验室）：填充毛细管柱和 Agilent 1100 HPLC 的使用 2. Dao-Fu Dao（Rabinovitch Lab）：填充毛细管柱，在 Waters nanoAcquity 和 Thermo LTQ-FT 仪器上进行样品分析并进行数据分析。 3. Peter Straub（范德比尔特）：与Bullseye一起进行数据分析。 4. Lea Starita（Fields Lab）：使用 Bullseye 和毛细管柱填料进行数据分析。 Gennifer Merrihew 提供的培训 1. MSDaPl / MacCoss 实验室管道帮助 - Evvie Vincow、Lea Starita、Jan Aagard、Melody Rynerson、Katrina Claw、Renee George、Angela Poole 2. Orbitrap 运行、样品准备讨论 - Evvie Vincow、Lea Starita 3. GelFree - Lea Starita、Andrew Stergachis、Chuck Witkowski、Jeremy Norris 4. LTQ 分析帮助、专栏制作帮助 - Melody Rynerson、Katrina Claw、Renee George、Jan Aagard、Angela Poole、Lea Starita 5. 古代蛋白质样品制备 - Ben Novak、Tom Kaye、Matthew Fitzgibbon、Pavel Pezner、Martin McIntosh 6. 同位素标记讨论 - Soledad Undurraga 7. TAP 标记的 FOXO 细胞系 - Matt Kaeberlein、Lara Shamieh