PROTEOMIC ANALYSIS OF EXTINCT SPECIES TO VALIDATE EVOLUTIONARY LINKS

对已灭绝物种进行蛋白质组学分析以验证进化联系

• 批准号: 8171400
• 负责人: Pavel A Pevzner
• 金额: $ 0.14万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171400
• 关键词: Collagen; Computer Retrieval of Information on Scientific Projects Database; Dinosaurs; Femur; Fossils; Funding; Grant; Hemoglobin; Institution; Link; Mass Spectrum Analysis; Methods; Morphologic artifacts; Preparation; Proteins; Proteomics; Research; Research Personnel; Resources; Sampling; Science; Source; Taxon; United States National Institutes of Health; Ursidae Family; bone; protein aminoacid sequence

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Researchers (Asara et. al., Science 2007) have started using mass spectrometry to identify proteins from fossils that have been preserved in extinct species such as T. rex and mastodon to provide evolutionary links with extant species. These recent studies have identified collagen proteins and a hemoglobin protein that contain peptide sequences that match to multiple different extant taxa. There is much controversy on whether these identified peptides sequences are valid or artifacts of contamination. We are currently analyzing six fossilized dinosaur bone samples, three sediment samples and a bone sample from the femur of a cave bear to see what proteins we can detect using our sample preparation and mass spectrometry methods.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 研究人员（Asara et al。，Science 2007）已开始使用质谱法来鉴定已保存在灭绝物种（例如T. Rex和Mastodon）中的化石中的蛋白质，以提供现有物种的进化联系。这些最近的研究已经确定了胶原蛋白和一种含有与多个不同现有分类群相匹配的肽序列的血红蛋白蛋白。关于这些鉴定出的肽序列是有效的还是污染的伪影，这是有争议的。 我们目前正在分析六个化石恐龙骨样品，三个沉积物样品和一个来自洞穴熊的股骨样品，以查看我们可以使用样品制备和质谱法检测到的蛋白质。