IDENTIFICATION OF NOVEL COMPONENTS IN TRANS-SNARE COMPLEX

跨圈套复合体中新成分的鉴定

• 批准号: 8171259
• 负责人: WILLIAM Tobey WICKNER
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171259
• 关键词: Biological Assay; Cancerous; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Family; Functional disorder; Funding; Grant; Guanosine Triphosphate Phosphohydrolases; Hereditary Disease; Hormones; Human; In Vitro; Institution; Life; Lysosomes; Membrane; Membrane Fusion; Normal Cell; Physiology; Process; Proteins; Reaction; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Source; United States National Institutes of Health; Vacuole; cell growth; neurotransmission; novel; reconstitution; tool; yeast genetics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Membrane fusion is the essence of cell growth (in both normal cells and cancerous cells), hormone secretion, and neurotransmission. Despite its fundamental character, the mechanisms of how membranes fuse are only slowly becoming understood. We study this process with vacuoles (lysosomes) from baker's yeast for the unparalleled power of experimental tools available: vacuoles are large and can be visualized in the living cell, their fusion can be essential or dispensable for viability (depending on the cell growth conditions), the genetics of yeast is well-advanced, we can assay vacuole fusion in vitro with quantitative, colorimetric assays, and the fusion reaction has been reconstituted with all-purified components. The mechanisms we discover, for the Rab-family GTPase, for its effector complex, and for SNAREs, are of general importance for understanding human physiology and pathophysiology, for these same proteins are essential for membrane fusion in humans.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 膜融合是细胞生长的本质（在正常细胞和癌细胞中），激素分泌和神经传递。 尽管具有根本的特征，但膜融合方式的机制只是逐渐被理解。 We study this process with vacuoles (lysosomes) from baker's yeast for the unparalleled power of experimental tools available: vacuoles are large and can be visualized in the living cell, their fusion can be essential or dispensable for viability (depending on the cell growth conditions), the genetics of yeast is well-advanced, we can assay vacuole fusion in vitro with quantitative, colorimetric assays, and the fusion reaction has been用全纯化的组件重构。 对于Rab-family GTPase，我们发现的机制对于其效应子络合物以及对鼻子的作用对于理解人类生理学和病理生理学至关重要，因为这些相同的蛋白质对于人类的膜融合至关重要。