DROSOPHILA TORC ASSOCIATED PROTEINS

果蝇 Torc 相关蛋白

基本信息

批准号：
8171243
负责人：
MARC R MONTMINY
金额：
$ 0.24万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-01 至 2011-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8171243
关键词：
CREB1 gene Cell Nucleus Computer Retrieval of Information on Scientific Projects Database Cues Cyclic AMP Cytoplasm Drosophila genus Equilibrium Family Fasting Funding Gene Expression Genes Genetic Glucagon Glucose Grant Hormonal Institution Intracellular Second Messenger Mammals Mediating Pancreas Proteins Proteomics Research Research Personnel Resources Role Second Messenger Systems Signal Transduction Source Starvation United States National Institutes of Health energy balance feeding insight programs promoter response tool

CREB1 gene Cell Nucleus Computer Retrieval of Information on Scientific Projects Database Cues Cyclic AMP Cytoplasm Drosophila genus Equilibrium Family Fasting Funding Gene Expression Genes Genetic Glucagon Glucose Grant Hormonal Institution Intracellular Second Messenger Mammals Mediating Pancreas Proteins Proteomics Research Research Personnel Resources Role Second Messenger Systems Signal Transduction Source Starvation United States National Institutes of Health energy balance feeding insight programs promoter response tool

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The intracellular second messenger cAMP functions as a starvation state signal, which mediates induction of catabolic programs in response to hormonal stimulation. Glucose balance in fasted mammals is maintained, for example, by induction of gluconeogenic genes by the cAMP responsive CREB coactivator TORC2 in response to elevations in circulating pancreatic glucagon. Sequestered in the cytoplasm under feeding conditions, TORC2 is dephosphorylated and translocated to the nucleus in response to cAMP signaling, whereupon it stimulates gene expression by associating with CREB over relevant promoters. To further dissect the role of the TORC family in energy balance, we will use proteomic and genetic tools to manipulate TORC activity in Drosophila. The identification of Drosophila TORC-associated proteins will provide new insights into the mechanisms by which this coactivator responds to environmental cues.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。细胞内的第二信使训练营充当饥饿状态信号，该信号介导了响应激素刺激的分解代谢程序的诱导。例如，通过cAMP反应性CREB共激活器TORC2诱导糖生成基因来维持禁食哺乳动物的葡萄糖平衡，以响应循环胰腺胰高血糖素的升高。在喂养条件下，Torc2在细胞质中隔离，被脱磷酸化并易于反应cAMP信号传导，然后通过与相关启动子与CREB相关联，从而刺激基因表达。为了进一步剖析Torc家族在能量平衡中的作用，我们将使用蛋白质组学和遗传工具来操纵果蝇中的Torc活性。果蝇相关蛋白的鉴定将提供有关该共激活因子对环境提示的机制的新见解。