CRYSTALLOGRAPHY OF CHROMATIN FACTOR/NUCLEOSOME COMPLEXES

染色质因子/核小体复合物的晶体学

• 批准号: 8169269
• 负责人: SONG TAN
• 金额: $ 0.7万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169269
• 关键词: Address; Base Pairing; Cells; Chromatin; Complex; Computer Retrieval of Information on Scientific Projects Database; Core Protein; Crystallography; DNA; Enzymes; Funding; Gene Expression Regulation; Genetic; Goals; Grant; Histones; Institution; Nucleosome Core Particle; Nucleosomes; Participant; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; reconstitution; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The DNA genetic information in our cells is packaged as chromatin. The nucleosome core particle is a fundamental repeating unit of chromatin and comprises about 150 base pairs of DNA spooled around a histone protein core. Recent studies show that chromatin is not simply a repressive structure that occludes DNA, but is instead an active participant in gene regulation that associates with multiple chromatin factors and enzymes. Despite the importance of such interactions, we currently lack a structural understanding of how chromatin factors or enzymes recognize the nucleosome. To address this problem, we have reconstituted complexes containing the nucleosome core particle and several chromatin factors or enzymes, and we have grown single crystals of a chromatin factor/nucleosome core particle complex. Our goal is to determine the three-dimensional structure of these chromatin factor/nucleosome complexes to understand how the cell recognizes and interacts with its genetic information assembled as chromatin.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们细胞中的DNA遗传信息被包装为染色质。核小体核心粒子是染色质的基本重复单元，包括大约150个基本对围绕组蛋白核心的DNA。最近的研究表明，染色质不仅是一种阻塞DNA的抑制性结构，而且是与多种染色质因子和酶相关的基因调节的积极参与者。尽管这种相互作用很重要，但我们目前缺乏对染色质因子或酶如何识别核小体的结构性理解。为了解决这个问题，我们已经重组了含有核小体核心颗粒和几种染色质因子或酶的复合物，并且我们已经种植了染色质因子/核小体核心粒子颗粒复合物的单晶。我们的目标是确定这些染色质因子/核小体复合物的三维结构，以了解细胞如何识别并与其遗传信息相互作用，以染色质组装。