MODEL PROTEIN PRODUCTION FOR TIME-RESOLVED 2D-ELDOR, PDS, AND MQC ESR

时间分辨 2D-ELDOR、PDS 和 MQC ESR 的蛋白质生产模型

• 批准号: 8172240
• 负责人: ELKA R GEORGIEVA
• 金额: $ 0.96万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172240
• 关键词: Biological Models; Calmodulin; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Coupled; DNA; Deer; Development; Funding; Grant; Housing; Institution; Methods; Modeling; Muramidase; Production; Proteins; Research; Research Personnel; Resources; Sampling; Source; Spectrum Analysis; Spin Labels; Time; United States National Institutes of Health; interest; Biological Models; Calmodulin; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Coupled; DNA; Deer; Development; Funding; Grant; Housing; Institution; Methods; Modeling; Muramidase; Production; Proteins; Research; Research Personnel; Resources; Sampling; Source; Spectrum Analysis; Spin Labels; Time; United States National Institutes of Health; interest

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Good model systems are needed to develop methods, which is certainly true of PDS. Long organic biradicals, polyradicals, and supramolecular assemblies were instrumental for development of DEER. However they are difficult to synthesize and require expertise of a good organic chemist, who should be interested in such collaborations. In our development of DQC method we could not rely on this but made a good use of few available biradicals with distances in the range of 10-30 ¿. But when we needed a longer distances, we had to reach to Gunnar Jeschke for the sample. However we did find that for example DNA duplexes or some proteins can also contribute good model systems and are relatively easy to produce in house in required quantities. For the project development on time-resolved 2D-ELDOR spectroscopy, coupled with stopped flow or continuous rapid flow, we require large amounts (up to grams) of spin-labeled model proteins. For example T4 Lysozyme and Calmodulin are relatively small and stable proteins that can be expressed in required quantities with a moderate effort. In general proteins and DNA could provide us with all required distances and a variety of spin configurations. Furthermore, they are much more relevant to our research.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 需要良好的模型系统来开发方法，这在PD上肯定是正确的。长的有机biradicals，多性恋和超分子组件对鹿的发育有用。但是，它们很难合成，并且需要一位良好的有机化学家的专业知识，他们应该对这种合作感兴趣。在我们开发DQC方法中，我们不能依靠这一点，而是很好地利用了少数可用的Biradicals，其距离在10-30»范围内。但是，当我们需要更长的距离时，我们必须到达Gunnar Jeschke进行样本。但是，我们确实发现，例如，DNA双链体或某些蛋白质也可以贡献良好的模型系统，并且相对容易以所需数量为单位。为了在时间分辨的2D欧洲光谱上进行项目开发，再加上停止流量或连续快速流动，我们需要大量的自旋标记模型蛋白的（最多）（最多）。例如，T4溶菌酶和钙调蛋白是相对较小且稳定的蛋白质，可以用现代努力以所需的数量表示。一般而言，蛋白质和DNA可以为我们提供所有必需的距离和各种自旋构型。此外，它们与我们的研究更加相关。