ASSESSING NITRIC OXIDE IN PLANT DEFENSE USING NO SPIN TRAPPING

不使用自旋捕获评估植物防御中的一氧化氮

• 批准号: 8172207
• 负责人: BORIS G DZIKOVSKI
• 金额: $ 0.03万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172207
• 关键词: Animals; Arabidopsis; Biology; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Detection; Development; Disease Resistance; Eukaryota; Flagellin; Flowers; Funding; Grant; Growth and Development function; Host Defense; Infiltration; Institution; Measurement; Methods; Microbe; Mouse-ear Cress; Nitric Oxide; Peptides; Physiological Processes; Plant Diseases; Plant Model; Plants; Play; Production; Prokaryotic Cells; Proteins; Pseudomonas syringae; Research; Research Personnel; Resources; Role; Signaling Molecule; Source; Spin Trapping; Stimulus; Stress; Suspension substance; Suspensions; Tobacco; United States National Institutes of Health; base; defense response; ferro-N-methyl-d-glucamide dithiocarbamate; interest; pathogen; response; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. NO acts as a signaling molecule in a wide range of physiological processes in both eukaryotes and prokaryotes. In plants, NO participates in growth, development, flowering, and response to biotic and abiotic stresses. It has been shown that nitric oxide plays an important role in plant disease resistance. However, unlike our appreciation of NO functioning in animals, the origin and the quantification of plant NO responses to stimuli remain unclear. A comprehensive understanding of NO biology is dependent on the development of reliable NO detection methods, and EPR represents a reliable tool in this respect. Stabilization of NO radical with spin trap complexes and its detection by ESR offer the advantage of being both selective and sensitive. Initial ESR study in ACERT using NO spin trap Fe(DETC)2 showed that the model plant Arabidopsis thaliana responds to the stress caused by infiltration with Pseudomonas syringae by more rapid and robust production of NO. The Dan Klessig group is interested in using lipophilic Fe(DETC)2 complex or hydrophilic Fe(MGD)2 spin traps to evaluate NO formation in the context of plant-microbe interactions in 1) plant cell suspensions (tobacco cells) elicited for NO production with cryptogein, a 10 kDa protein secreted by the oomycete Phytophtora cryptogea that induces host defense responses, and 2) in Arabidopsis plants infected with either a bacterial pathogen (Pseudomonas syringae) or a peptide elicitor corresponding to the conserved domain of bacterial flagellin. We currently use 15N substitution to exploit the ability of the ESR measurement to distinguish between 14NO and 15NO, and we are confident that an ESR-based approach will help to elucidate the origin(s) of this radical in planta.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在真核生物和原核生物中，在广泛的生理过程中，没有任何充当信号分子。在植物中，没有参与生长，发育，开花以及对生物和非生物胁迫的反应。 已经表明，一氧化氮在植物性抗病性中起重要作用。但是，与我们对动物无功能的欣赏不同，植物的起源和量化无反应尚不清楚。 对没有生物学的全面理解取决于可靠的无检测方法的发展，而EPR在这方面代表了可靠的工具。 没有自旋陷阱复合物及其通过ESR检测的无自由基的稳定，这是选择性和敏感的优势。最初使用无自旋陷阱Fe（detc）2在ACERT中进行的ESR研究表明，模型植物拟南芥塔利亚纳（Thaliana）响应了丁香假单胞菌浸润所带来的压力，这是通过更快且强大的NO产生。 The Dan Klessig group is interested in using lipophilic Fe(DETC)2 complex or hydrophilic Fe(MGD)2 spin traps to evaluate NO formation in the context of plant-microbe interactions in 1) plant cell suspensions (tobacco cells) elicited for NO production with cryptogein, a 10 kDa protein secreted by the oomycete Phytophtora cryptogea that induces host defense responses, 2）在感染细菌病原体（假单胞菌）或与细菌鞭毛蛋白保守结构域相对应的肽的植物中。目前，我们使用15N替代来利用ESR测量区分14NO和15NO的能力，并且我们有信心基于ESR的方法将有助于阐明Planta中这种激进的起源。