PNPASE COMPLEX

PNP酶复合物

• 批准号: 8168586
• 负责人: Wen-Hwa Lee
• 金额: $ 1.08万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168586
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Human Genome; In Vitro; Institution; Knowledge; Mitochondria; Mitochondrial RNA; Monitor; Proteins; RNA Degradation; Recombinants; Research; Research Personnel; Resources; Role; Source; Testing; United States National Institutes of Health; Work; degradosome; mitochondrial dysfunction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There is a readily recognizable PNPase-like molecule in the human genome, which localizes to mitochondria, and its deficiency leads to mitochondrial dysfunction. Importantly, we have found a direct interaction between recombinant SUV3 and PNPase in vitro. We plan to further examine their interaction in detail to test whether SUV3 works in concert with PNPase in monitoring mitochondrial RNA degradation. Are there other proteins in the degradosome together with SUV3/PNPase? If yes, what are their roles in RNA degradation? Isolating and identifying the SUV3/PNPase complex from mitochondria are critical steps to advance our current knowledge of the mammalian mitochondrial degradosome.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 人类基因组中有一个容易识别的PNPase样分子，该分子定位于线粒体，其缺乏会导致线粒体功能障碍。重要的是，我们在体外发现了重组SUV3与PNPase之间的直接相互作用。我们计划进一步检查它们的相互作用，以测试SUV3是否与PNPase一起监测线粒体RNA降解。降解体中还有其他蛋白质以及SUV3/PNPase吗？如果是，它们在RNA降解中的作用是什么？从线粒体中隔离和识别SUV3/PNPase复合物是提高我们当前对哺乳动物线粒体降解体了解的关键步骤。