BINDING OF CADMIUM AND ZINC BY A CD/ZN ATPASE INVOLVED IN METAL HYPERACCUMULATIO

涉及金属超积累的 CD/ZN ATP 酶对镉和锌的结合

• 批准号: 8170251
• 负责人: WOLFRAM MEYER-KLAUCKE
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170251
• 关键词: ATP phosphohydrolase; Area; Basic Science; Binding; Biochemistry; Biophysics; Cadmium; Computer Retrieval of Information on Scientific Projects Database; Copper; Disease; Family member; Funding; Genes; Grant; Heavy Metals; Hepatolenticular Degeneration; Human; Institution; Lead; Ligands; Metals; Mining; Plant Physiology; Plant Roots; Plants; Proteins; Pump; Research; Research Personnel; Resources; Site; Soil; Source; United States National Institutes of Health; Zinc; disease-causing mutation; interest; nutrition; operation; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Heavy metal uptake in plants is an important research area not only in the sense of basic research, but also because of its impact on human nutrition and its application for the phytoremediation of contaminated soils. For the latter task, as well as for the commercial extraction of metals from naturally metal-rich soils that are, however, unsuitable for conventional mining operations, hyperaccumulator plants are of primary importance. These plants are able to accumulate several percent of metals in the dry mass of their above-ground parts; the unusual physiology of these plants is a topic of intensive worldwide research efforts. One of the presently most interesting topics in this area of research are the biochemistry and biophysics of transporters that lead metal from the roots to their final storage site in the shoot. In the current project we successfully purified a Cd/Zn ATPase (TcHMA4) from hyperaccumulator roots in its native state (Parameswaran et al., 2007) and we now want to further investigate which ligands in TcHMA4 bind metal (in this case cadmium). The protein belongs to the P1B-type ATPases that are present in humans as well and several members of this family of pumps are involved in important diseases like the Menkes and Wilsons diseases that are caused by mutation in copper ATPases. While the genes are known, only little is known about biochemistry and biophysics of P1B-type ATPases as the purification is difficult to achieve.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 重金属在植物中的吸收不仅是基础研究意义上的重要研究领域，而且还因为它对人类营养的影响及其在受污染土壤的植物修复中的应用。对于后一项任务，以及从天然富含金属富含土壤的金属的商业提取，这些金属不适合常规采矿作业，高积累植物的主要重要性至关重要。这些植物能够在其地面部分干燥质量中积聚数％的金属。这些植物的异常生理是全球研究工作的一个话题。目前在这一研究领域中最有趣的主题之一是转运蛋白的生物化学和生物物理学，它们将金属从根部到芽中的最终存储地点。在当前的项目中，我们从其天然状态的高含量的根中成功纯化了CD/Zn ATPase（TCHMA4）（Parameswaran等，2007），现在我们想进一步研究TCHMA4中的哪种配体结合金属（在这种情况下为镉）。该蛋白质也属于人类中存在的P1B型ATPases，该泵家族的几个成员参与了重要疾病，例如由铜ATPases突变引起的Menkes和Wilsons疾病。尽管这些基因是已知的，但对于P1B型ATPases的生物化学和生物物理学知之甚少，因为纯化很难实现。