STRUCTURAL STUDIES ON SOLUBLE AND MEMBRANE BOUND PROTEINS INVOLVED IN OXIDATIVE

参与氧化的可溶性和膜结合蛋白的结构研究

• 批准号: 8170215
• 负责人: AINA COHEN
• 金额: $ 0.17万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170215
• 关键词: Anabolism; Binding Proteins; Biochemical Pathway; Cellular Membrane; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Dihydroxyacetone Phosphate; Electron Transport; Enzymes; Funding; Glycerol; Glycerol Kinase; Glycerol-3-Phosphate Dehydrogenase; Glycerophospholipids; Grant; Homologous Gene; Institution; Ion Channel; Mediating; Membrane; Membrane Proteins; Metabolic Pathway; Mitochondria; Pathway interactions; Play; Production; Reaction; Recycling; Research; Research Personnel; Resources; Respiration; Respiratory Chain; Role; Source; United States National Institutes of Health; alpha-glycerophosphoric acid; driving force; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The glycerol metabolic pathway is a fundamental biochemical pathway, crucial to the biosynthesis of essential cellular components and for energy production. Functionally, the uptake of glycerol relies on the cytoplasmic glycerol kinase (GlpK), which provides the chemical driving force for the non-ATP dependent uptake of glycerol. Enhanced glycerol uptake is mediated through the membrane-channel formed by the glycerol facilitator (GlpF). Another membrane protein, the glycerol-3-phosphate (G3P) dehydrogenase (GlpD), oxidizes glycerol-3-phosphate to dihydroxyacetone phosphate, which can be utilized in energy production via the glycolytic pathway and provides reducing equivalents to Enzyme III of the mitochondrial respiratory chain. Additionally, the GlpD can synthesize G3P for the biosynthesis of the glycerophospholipids that are incorporated into cellular membranes. A soluble homolog of the GlpD is the glycerophosphate oxidase (GlpO), which catalyzes the same reaction of oxidizing G3P to DHAP and also plays a role in respiration through the G3P shuttle that recycles reducing equivalents to the mitochondrial electron transport chain. Consequently, all of these enzymes play highly important roles in biosynthetic pathways and in energy production. Comprehensive structural studies are proposed.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 甘油代谢途径是一种基本的生化途径，对必需细胞成分的生物合成至关重要。在功能上，甘油的摄取依赖于细胞质甘油激酶（GLPK），该甘油激酶（GLPK）为非ATP依赖性甘油提供了化学驱动力。增强的甘油吸收是通过甘油促进剂（GLPF）形成的膜通道介导的。另一种膜蛋白是甘油-3-磷酸（G3P）脱氢酶（GLPD），将甘油-3-磷酸氧化为二羟基二乙酮磷酸，可通过糖酵解途径的糖化途径生产，并为浓度降低了nzemeiii iiii III III III III III III III III III IIII的磷酸化途径。此外，GLPD可以合成G3P，以掺入细胞膜中的甘油磷脂的生物合成。 GLPD的可溶性同源物是甘油磷酸氧化酶（GLPO），它催化G3P对DHAP的氧化反应相同，并且还通过G3P穿梭在呼吸中发挥作用，通过G3P穿梭将回收物减少到线粒体电子传输链中。因此，所有这些酶在生物合成途径和能源生产中都起着非常重要的作用。 提出了全面的结构研究。