OPTIMIZATION OF ETD TECHNIQUE ON THE LTQ ORBITRAP

LTQ Orbitrap 上 ETD 技术的优化

• 批准号: 8169794
• 负责人: ALMA L BURLINGAME
• 金额: $ 2.12万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169794
• 关键词: Amino Acids; Computer Retrieval of Information on Scientific Projects Database; Detection; Development; Dissociation; Electron Transport; Funding; Grant; Institution; Ions; Peptides; Post-Translational Protein Processing; Proteins; Research; Research Personnel; Resources; Side; Source; Techniques; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Electron transfer dissociation (ETD) can be used to provide unique and complementary fragment ions when compared to other dissociation techniques, such as collision induced dissociation (CID). Weak side chain bonds are observed at phosphorylated amino acids in peptides are often lost during CID, but preserved during ETD. This makes the technique useful for the detection and analysis of posttranslational modifications in peptides and intact proteins. This project is devoted to the development and improvement of ETD for such applications.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 与碰撞诱导解离 (CID) 等其他解离技术相比，电子转移解离 (ETD) 可用于提供独特且互补的碎片离子。 在肽中的磷酸化氨基酸处观察到弱侧链键通常在 CID 期间丢失，但在 ETD 期间保留。 这使得该技术可用于检测和分析肽和完整蛋白质的翻译后修饰。 该项目致力于开发和改进此类应用的 ETD。