OPTIMIZATION OF ETD TECHNIQUE ON THE LTQ ORBITRAP

LTQ Orbitrap 上 ETD 技术的优化

• 批准号: 8169794
• 负责人: ALMA L BURLINGAME
• 金额: $ 2.12万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169794
• 关键词: Amino Acids; Computer Retrieval of Information on Scientific Projects Database; Detection; Development; Dissociation; Electron Transport; Funding; Grant; Institution; Ions; Peptides; Post-Translational Protein Processing; Proteins; Research; Research Personnel; Resources; Side; Source; Techniques; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Electron transfer dissociation (ETD) can be used to provide unique and complementary fragment ions when compared to other dissociation techniques, such as collision induced dissociation (CID). Weak side chain bonds are observed at phosphorylated amino acids in peptides are often lost during CID, but preserved during ETD. This makes the technique useful for the detection and analysis of posttranslational modifications in peptides and intact proteins. This project is devoted to the development and improvement of ETD for such applications.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 与其他解离技术相比，电子转移解离（ETD）可用于提供独特的和互补的片段离子，例如碰撞诱导的解离（CID）。 在CID期间，在肽中磷酸化的氨基酸处观察到弱的侧链键，但在ETD期间保存。 这使得该技术可用于检测和分析肽和完整蛋白质中翻译后修饰。 该项目致力于此类应用的ETD的开发和改进。