STRUCTURE/FUNCTION AND PROTEIN-PROTEIN INTERACTION ANALYSIS IN HG DETOXIFICATION

HG 解毒中的结构/功能和蛋白质-蛋白质相互作用分析

• 批准号: 8169723
• 负责人: Susan Mary Miller
• 金额: $ 4.95万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169723
• 关键词: Bacterial Proteins; Chemicals; Complex; Computer Retrieval of Information on Scientific Projects Database; Drug Metabolic Detoxication; Enzymes; Funding; Goals; Grant; Individual; Institution; Ions; Membrane Transport Proteins; Mercury; Metals; Mutate; Pathway interactions; Property; Protein Analysis; Proteins; Proteolysis; Research; Research Personnel; Resources; Role; Site-Directed Mutagenesis; Source; Structure; United States National Institutes of Health; oxidation; physical property; protein protein interaction; protein structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this project we are investigating the role of protein-protein interactions in the mechanism of metal ion transfers between the key proteins of the bacterial detoxification pathway for inorganic mercury and organomercurials and elucidating the chemical and physical properties embedded in the protein structures that facilitate the directed transfer of Hg(II) to the essential enzyme for reductive detoxification. The structures of four components of the pathway have been determined and the structure of an integral membrane transport protein is being pursued to facilitate structure/function analysis of the proteins. Together with the structural information we are using site-directed mutagenesis to evaluate the effects of altered structural features on the individual properties and functions of the several proteins and on their interactions. The goal of the studies is to reveal the features of the proteins that are essential from an evolutionary perspective for this detoxification pathway to be effective. Mass spec has proved invaluable in verifying oxidation and proteolysis problems with several of our mutated enzymes and in verifying formation of metal ion complexes that we have used in our structural studies.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 In this project we are investigating the role of protein-protein interactions in the mechanism of metal ion transfers between the key proteins of the bacterial detoxification pathway for inorganic mercury and organomercurials and elucidating the chemical and physical properties embedded in the protein structures that facilitate the directed transfer of Hg(II) to the essential enzyme for reductive detoxification.已经确定了途径的四个组成部分的结构，并正在追求积分膜转运蛋白的结构，以促进蛋白质的结构/功能分析。 与结构信息一起，我们使用位置定向的诱变来评估结构特征改变对几种蛋白质的单个特性和功能的影响及其相互作用。 研究的目的是揭示蛋白质的特征，从进化的角度来看，这是该排毒途径有效的。 事实证明，质量规格在验证我们的几种突变酶以及验证我们在结构研究中使用的金属离子复合物的形成方面验证氧化和蛋白水解问题是无价的。