BIOACTIVE LIPIDS IN THE HUMAN FOOD SUPPLY

人类食品中的生物活性脂质

• 批准号: 8169802
• 负责人: ERIC J HAAS-STAPLETON
• 金额: $ 0.53万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169802
• 关键词: Candida albicans; Categories; Cells; Computer Retrieval of Information on Scientific Projects Database; Dinoprostone; Food; Food Supply; Funding; Goals; Grant; Human; Immune system; Inflammation; Institution; Lipids; Mass Spectrum Analysis; Outcome Measure; Pathway interactions; Pharmacologic Substance; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Signaling Molecule; Source; United States National Institutes of Health; Vaccines; fungus; lipid biosynthesis; liquid chromatography mass spectrometry; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bioactive lipids, such as prostaglandin E2 (PGE2), are signaling molecules generated by cells of the immune system to regulate inflammation in humans. Bioactive lipids are also biosynthesized by fungi, such as the pathogen Candida albicans and the saprophyte Saccharomyces cerevisiae. The latter is employed to manufacture a wide range of foods, pharmaceuticals and vaccines. We aim to identify the categories of products consumed by humans that contain bioactive lipids produced by S. cerevisiae and define the biosynthetic pathway(s) used by the fungus to produce bioactive lipids. Understanding how fungi generate bioactive lipids will inform of strategies for limiting bioactive lipid biosynthesis during product manufacture. The proposed research is initially focused upon PGE2 because it is one of the better-understood bioactive lipids and is produced by S. cerevisiae. The outcome measure of this study will be determining whether bioactive lipids are absent or present in select products manufactured using S. cerevisiae. The UCSF Mass Spectrometry Facility can assist in obtaining our research goals by analyzing extracted lipids from select products for the presence of bioactive lipids such as PGE2, using liquid chromatography-mass spectrometry (LC-MS).

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 生物活性脂质，例如前列腺素 E2 (PGE2)，是免疫系统细胞产生的信号分子，可调节人体炎症。生物活性脂质也可由真菌生物合成，例如病原体白色念珠菌和腐生菌酿酒酵母。后者用于生产各种食品、药品和疫苗。 我们的目标是确定人类消费的含有酿酒酵母产生的生物活性脂质的产品类别，并确定真菌用于生产生物活性脂质的生物合成途径。 了解真菌如何产生生物活性脂质将为在产品制造过程中限制生物活性脂质生物合成的策略提供信息。 拟议的研究最初集中在 PGE2 上，因为它是人们更了解的生物活性脂质之一，由酿酒酵母产生。这项研究的结果测量将确定使用酿酒酵母制造的选定产品中是否不存在或存在生物活性脂质。 UCSF 质谱设施可以使用液相色谱-质谱 (LC-MS) 分析从选定产品中提取的脂质中是否存在 PGE2 等生物活性脂质，从而帮助实现我们的研究目标。