IN VIVO T2, T1R, AND SODIUM IMAGING OF ARTICULAR CARTILAGE AT 30T

30T 下关节软骨的体内 T2、T1R 和钠成像

• 批准号: 8169863
• 负责人: Garry E Gold
• 金额: $ 1.85万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169863
• 关键词: Age; Anatomy; Anterior; Area; Cartilage; Cartilage Matrix; Collagen; Computer Retrieval of Information on Scientific Projects Database; Computer software; Custom; Degenerative polyarthritis; Deposition; Detection; Early Diagnosis; Feasibility Studies; Frequencies; Funding; Grant; Healthcare; Image; Institution; Knee bone; Lateral; Location; Magnetic Resonance Imaging; Maps; Measurement; Measures; Medial; Methods; Physiologic pulse; Proteoglycan; Radio; Recording of previous events; Relaxation; Research; Research Personnel; Resolution; Resources; Retinal Cone; Scanning; Signal Transduction; Slice; Sodium; Source; Surface; Techniques; Thick; Time; United States National Institutes of Health; arm; articular cartilage; drug discovery; healthy volunteer; in vivo; knee pain; therapy development; volunteer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Detection of matrix changes of articular cartilage in early osteoarthritis is important for drug discovery. We studied the fesability of comparing T2, T1r, and sodium imaging in healthy volunteers and one subject with knee pain at 3.0T. T2 mapping was done with a T2-prepared spiral method. T1r imaging was performed using a continious spin-lock pulse at several locking frequencies. Sodium imaging was done with a custom surface coil and a short echo-time 3D cones trajectory. Our results indicate it is feasible to compare these methods in vivo at 3.0T for early cartilage matrix changes.Introduction: Early detection of proteoglycan depletion in cartilage is important in development of treatments for osteoarthritis. T1r imaging, or relaxation of spins under the influence of a radio-frequency field, has been shown to be sensitive to changes in the cartilage matrix . T2 mapping is thought to reflect changes in the collagen matrix of cartilage. Sodium imaging has also been used to measure proteoglycan content in cartilage. We studied the feasibility of using these techniques in vivo at 3.0T. Methods: Four volunteers (ages 34-43) were imaged in the axial plane at 3.0T on a GE Signa MRI (GE Healthcare, Milwaukee, WI) using 3-inch coil. One volunteer had a history of anterior knee pain. Measurements of T1r were made with a continuous RF spin locking pulse at the same anatomic locations with spin lock frequencies of 300, 500, 700 and 1000 Hz. The maximum spin-lock frequency in the continuous RF sequence was limited by RF power deposition limits. T2 relaxation time at the same location was measured using a T2-prepared spiral sequence. The T1r and CPMG T2 sequences had identical imaging parameters: TR of 2000 ms, 14 spiral arms, 4096 points, and bandwidth +125 kHz. In-plane resolution was 0.7 mm with a 16 cm FOV, 4 mm slice thickness. A single slice through the patella cartilage was acquired in 5 minutes with two signal averages. The T1r sequences acquired 4 spin lock times (TSL) of 3, 15, 35, and 80 ms. The CPMG T2 sequence acquired 6 echoes at approximately 6, 17, 28, 49, 71 and 92 ms. Sodium imaging was performed with a custom 3-inch surfaces coil and a short echo time "cones" trajectory . Sodium acquisitions were obtained at a voxel size of 1.25x1.25x4 mm. Parameters for the scan were: TR/TE = 50/0.6 ms, FOV = 16x16x12.8cm, matrix = 128x128x32, readout time = 8 ms, alpha = 70 deg, and 16 averages for a total scan time of 17 min 8 s. T1p and T2 relaxation times for each subject were measured in 10 cartilage locations on the medial and lateral patella facets. Sodium SNR was also measured in 10 locations on the medial and lateral facets, and in an area of increased relaxation times in the subject with knee pain. Relaxation measurements and maps were created using Osirix software .

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 检测早期骨关节炎中关节软骨的基质变化对于药物发现很重要。 我们研究了在健康志愿者中比较T2，T1R和钠成像的典型性，而一名受试者在3.0T时膝盖疼痛。用T2预先准备的螺旋方法进行T2映射。使用在几个锁定频率下使用持续的自旋脉冲进行T1R成像。 使用定制的表面线圈和短回波时间3D锥轨迹进行钠成像。 我们的结果表明，可以在3.0 t的体内进行早期软骨基质变化的体内方法进行比较。引言：软骨中蛋白聚糖耗竭的早期检测对于开发骨关节炎的治疗很重要。 在射频场的影响下，T1R成像或旋转的松弛已被证明对软骨基质的变化很敏感。 T2映射被认为反映了软骨的胶原蛋白基质的变化。 钠成像也已用于测量软骨中的蛋白聚糖含量。 我们研究了在3.0 t中在体内使用这些技术的可行性。 方法：使用3英寸线圈，在3.0T的轴向平面（3.0T）中，在3.0吨的轴向平面上成像了四名志愿者（34-43岁）。 一名志愿者有前膝疼痛的病史。用连续的RF旋转锁定脉冲在相同的解剖位置进行T1R的测量，其自旋锁定频率为300、500、700和1000 Hz。 连续RF序列中的最大自旋频率受RF功率沉积极限的限制。 使用T2制备的螺旋序列测量相同位置的T2松弛时间。 T1R和CPMG T2序列具有相同的成像参数：2000毫秒的TR，14个螺旋臂，4096点和带宽+125 kHz。 平面分辨率为0.7毫米，厚度为16 cm，切片厚度为16 cm。 在5分钟内获得两个信号平均值，在5分钟内获取了一个穿过the骨软骨的切片。 T1R序列获得了4个3、15、35和80 ms的4个自旋锁定时间（TSL）。 CPMG T2序列在大约6、17、28、49、71和92 ms处获得了6个回声。 使用定制的3英寸表面线圈和短回波时间“锥”轨迹进行钠成像。钠采集以1.25x1.25x4 mm的体素尺寸获得。 扫描的参数为：TR/TE = 50/0.6 ms，FOV = 16x16x12.8cm，矩阵= 128x128x32，读取时间= 8 ms，Alpha = 70 ver = 70 ver和16平均值，总扫描时间为17 min 8 s。 每个受试者的T1P和T2松弛时间在内侧和外侧骨面上的10个软骨位置测量。 还在内侧和侧面的10个位置测量了SNR钠，并且在受试者膝盖疼痛的情况下增加了松弛时间的区域。 使用Osirix软件创建了放松测量和地图。