Fatty Acid and Eicosanoid Analysis Core/Robert C. Murphy

脂肪酸和类二十烷酸分析核心/Robert C. Murphy

• 批准号: 8007062
• 负责人: FLOYD H CHILTON
• 金额: $ 13.45万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8007062
• 关键词: Arachidonate 15-Lipoxygenase; Arachidonic Acids; Arts; Biochemical; Biological; Biological Assay; Biological Models; Blood capillaries; Botanicals; Cells; Coupled; Development; Eicosanoids; Eicosapentaenoic Acid; Event; Fatty Acids; Health; Individual; Investigation; Knowledge; Lead; Leukotrienes; Lipids; Liquid Chromatography; Mass Fragmentography; Mass Spectrum Analysis; Measures; Oils; Pathway interactions; Polyunsaturated Fatty Acids; Preventive; Prostaglandin-Endoperoxide Synthase; Prostaglandins; Research Project Grants; Sampling; Sensitivity and Specificity; Techniques; Technology; base; capillary; ionization; liquid chromatography mass spectrometry; research study; tandem mass spectrometry; tool

It has become quite clear that in order to understand the intricacies of biochemical events taking place within cells that lead to the synthesis of eicosanoids such as prostaglandins and leukotrienes, one must have a means by which one can measure virtually all products of these pathways. The development of analytical techniques have kept pace with our understanding of these needs so that extraordinarily powerful tools are now available. Mass spectrometry has emerged as the technique of choice based on sensitivity and specificity demanded by the projects proposed in this application. Advances in electro-spray ionization and tandem mass spectrometry when coupled to liquid chromatography (LC/MS), has made it possible to carry out 30 targeted eicosanoids, or more, in a single LC/MS/MS experiment. Capillary gas chromatography/mass spectrometry (GC/MS) remains an unparalleled technique in its capability to identify and quantitate fatty acids (as many a 20 fatty acids in a single experiment). Based on this state of the art technology, the major specific aims of this core are to provide state of the art assays for target lipids on a routine basis to facilitate investigations in each of the individual research projects within the Center proposal. Specifically: 1) liquid chromatography/mass spectrometry (LC/MS) and tandem mass spectrometry (LC/MS/MS) will be used to quantitate metabolites of arachidonic acid and eicosapentaenoic acid by the cyclooxygenase and 5- and 12/15-lipoxygenase pathway and 2) Gas chromatography/mass spectrometry (GC/MS) will be used to quantitate and identify fatty acids, including polyunsaturated fatty acids in biological extracts.

已经很清楚的是，为了了解发生生化事件的复杂性 在导致类花生酸合成的细胞内，例如前列腺素和白细胞，必须具有 几乎可以测量这些途径的所有产品的手段。分析的发展 技术与我们对这些需求的理解保持同步，以便非常强大的工具是 现在可用。质谱已成为基于灵敏度和 本申请中提出的项目要求的特异性。电喷雾电离和 连接到液相色谱（LC/MS）时，串联质谱法已成为可能 在单个LC/MS/MS实验中，将30个靶向类花生素（或更多）。毛细管气 色谱/质谱法（GC/MS）仍然是一种无与伦比的技术 并定量脂肪酸（在一个实验中，许多20个脂肪酸）。基于这种艺术状态 技术是该核心的主要特定目的是为目标脂质提供最先进的测定法 日常基础，以促进中心提案中每个单个研究项目的调查。 具体：1）液相色谱/质谱法（LC/MS）和串联质谱法 （LC/MS/MS）将用于定量用蛛网膜酸和二糖烯酸代谢物的代谢产物。 环氧酶以及5-和12/15-脂氧合酶途径和2）气相色谱/质谱法 （GC/MS）将用于定量和识别脂肪酸，包括生物学中的多不饱和脂肪酸 提取物。