Flow Cytometry

流式细胞仪

• 批准号: 7930103
• 负责人: Kent J. Weinhold
• 金额: $ 18.83万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-15 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7930103
• 关键词: AIDS Vaccines; AIDS therapy; Academic Medical Centers; Acquired Immunodeficiency Syndrome; Animals; Arts; Basic Science; Cells; Clinical Research; Color; Core Facility; Development; Flow Cytometry; Fluorescence-Activated Cell Sorting; HIV; HIV-1; Housing; Human; Immunologic Monitoring; Informatics; Investigation; Laboratories; Life; Link; Process; Research; Research Personnel; Scientist; Sorting - Cell Movement; Specimen; Technology; Translational Research; base; global health; member; novel; pathogen; research facility; tool

CoreC The proposed Duke CFAR Flow Cytometry Core Facility will a) provide state-of-the-art, multi-color, BSL-3 fluorescence activated cell sorting support for basic research or translational investigations conducted by Duke CFAR members, including sorting HIV-1 infected cells, b) provide state-of-the-art, multi-color, BSL-2* flow cytometric analytical support for basic, translational, and clinical research investigations conducted by Duke CFAR members, and c) enhance the scientific approach used by Duke CFAR Investigators by educating the Core User base in more effective ways of utilizing the state-of-the-art tools offered by the Duke CFAR Flow Cytometry Core Facility, as well as to provide programmatic advancement for Managers and Operators of the CFAR Flow Cytometry Core Facility. The BSL-3 Sorting Component Laboratory will house the only flow cytometer at Duke University Medical Center capable of sorting live, unfixed human or animal derived cells, infected with HIV-1 or equivalent pathogens, and will prove an invaluable asset to the overall scope of the proposed Duke CFAR Flow Cytometry Core Facility. The Duke CFAR Flow Cytometry Core will be the only flow cytometry facility at Duke capable of providing polychromatic and flow informatic support for HIV and HIV-related translational research at Duke. The proposed Duke CFAR Flow Cytometry Core Facility will be comprised of two Component Laboratories, both housed in biocontainment laboratories within the SORF & Global Health Buildings, respectively. The Component Laboratories will be closely linked physically, administratively, and scientifically, in order to form a highly interactive and unified Duke CFAR Flow Cytometry Core Facility. The BSL-2* Analytical Component Laboratory will provide a broad range of polychromatic flow cytometry applications to CFAR investigators and, together with the BSL-3 Sorting Component Laboratory, comprise the only flow cytometry research facility on campus that routinely processes, sorts, and analyzes HIV-1 infected specimens.

核心C 拟建的杜克 CFAR 流式细胞术核心设施将 a) 为杜克 CFAR 成员进行的基础研究或转化研究提供最先进的多色 BSL-3 荧光激活细胞分选支持，包括对 HIV-1 感染者进行分选b) 为杜克大学 CFAR 成员进行的基础、转化和临床研究提供最先进的多色 BSL-2* 流式细胞分析支持，以及 c) 增强杜克大学使用的科学方法CFAR 研究人员通过更有效的方式教育核心用户群使用杜克大学 CFAR 流式细胞术核心设施提供的最先进的工具，并为 CFAR 流式细胞术核心的管理者和操作员提供程序化的进步设施。 BSL-3 分选组件实验室将配备杜克大学医学中心唯一的流式细胞仪，能够对感染 HIV-1 或同等病原体的活的、未固定的人类或动物来源的细胞进行分选，并将证明对整个范围的宝贵资产。拟议的杜克 CFAR 流式细胞术核心设施。杜克大学 CFAR 流式细胞术核心将是杜克大学唯一能够为杜克大学的 HIV 和 HIV 相关转化研究提供多色和流式信息支持的流式细胞术设施。拟议的杜克 CFAR 流式细胞术核心设施将由两个组件实验室组成，两个实验室分别位于 SORF 和全球健康大楼内的生物防护实验室内。各组成实验室将在物理、管理和科学上紧密联系，以形成高度互动和统一的杜克CFAR流式细胞术核心设施。 BSL-2* 分析元件实验室将为 CFAR 研究人员提供广泛的多色流式细胞术应用，并与 BSL-3 分选元件实验室一起构成校园内唯一的流式细胞术研究设施，可进行常规处理、分类和分析HIV-1 感染的标本。