DENDRITIC CELLS & IMMUNE RESPONSE IN CORNEAL TISSUE

树突状细胞

• 批准号: 8168425
• 负责人: SALOMON ESQUENAZI
• 金额: $ 17.28万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168425
• 关键词: Antigen-Presenting Cells; Apoptosis; Astigmatism; Cell physiology; Cells; Chemotaxis; Cicatrix; Clinical Research; Computer Retrieval of Information on Scientific Projects Database; Cornea; Dendritic Cells; Epithelial; Event; Experimental Models; Extracellular Matrix; Fibroblasts; Funding; Goals; Grant; Healed; Hyperopia; Immune response; Impaired wound healing; Impairment; Inflammation; Inflammation Mediators; Inflammatory; Institution; Keratectomy, Subepithelial, Laser-Assisted; Lead; Modeling; Mus; Myofibroblast; Myopia; Operative Surgical Procedures; Patients; Procedures; Process; Research; Research Personnel; Resources; Signal Transduction; Site; Source; Surgical Injuries; Testing; Tissues; United States National Institutes of Health; Up-Regulation; Vision; cytokine; eye dryness; healing; macrophage; migration; ocular surface; prevent; wound

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Lamellar corneal refractive surgery (procedures such as PRK, LASEK, LAS IK and CK that are commonly performed to correct myopia, hyperopia and astigmatism) leads to keratocyte apoptosis in the stroma adjacent to the epithelial surgical injury. This apoptosis, in turn, leads to activation of adjacent keratocytes, transformation and migration of fibroblasts and myofibroblasts, and alterations of the extracellular matrix. These events include an up-regulation of cytokine release and chemotaxis of inflammatory cells to the wound site. Although most patients heal without complications, some develop a state called "dry eye" characterized by a dry ocular surface and loss of trophic factors that leads to epithelial breakdown and increased inflammation which can, in some cases lead to abnormal scarring and vision impairment. The different signals that lead to normal or abnormal healing of the cornea are poorly understood. Our hypothesis is that "differences in macrophage or dendritic cell function may in part determine whether there is an adequate healing of the cornea or there is an impaired healing process after refractive surgery". We will therefore focus our studies on the characterization of macrophages and dendritic cells (DC), both antigen presenting cells and the inflammatory mediators induced by refractive surgery, in models of normal or abnormal healing. The proposed studies use PRK in mouse cornea as the experimental model. The long-term goal of this research is to understand the mechanisms leading to abnormal healing of the cornea after surgery, and develop clinical studies to test whether modulation of the immune response can prevent or reverse abnormal healing.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 层状角膜折射手术（通常进行以纠正近视，超局局和散光为诸如PRK，Lasek，Las IK和CK等方法会导致与上皮外科手术损伤相邻的基质中的角膜细胞凋亡。反过来，这种凋亡导致相邻角化细胞的激活，成纤维细胞和肌纤维细胞的转化和迁移以及细胞外基质的改变。这些事件包括细胞因子释放的上调以及炎症细胞对伤口部位的趋化性。尽管大多数患者愈合而没有并发症，但有些患者会发展出一种称为“干眼症”的状态，其特征是干眼表面和营养因子的丧失，导致上皮崩溃和炎症增加，在某些情况下会导致异常的疤痕和视力障碍。了解角膜正常或异常愈合的不同信号知之甚少。我们的假设是：“巨噬细胞或树突状细胞功能的差异可能部分决定了角膜的愈合，还是折射手术后的愈合过程受损”。因此，在正常或异常愈合的模型中，我们将把研究重点放在巨噬细胞和树突状细胞（DC）的表征上，抗原呈递细胞和折射手术诱导的炎症介质。拟议的研究使用小鼠角膜中的PRK作为实验模型。这项研究的长期目标是了解导致手术后角膜异常愈合的机制，并开发临床研究以测试免疫反应的调节是否可以预防或反向异常愈合。