INBRE:UPR-RPC: DNA SEQUENCING AND MULTI-LOCUS GENOTYPING FACILITIES

INBRE：UPR-RPC：DNA 测序和多位点基因分型设施

• 批准号: 8167841
• 负责人: TOMAS HRBEK
• 金额: $ 5万
• 依托单位: UNIVERSITY OF PUERTO RICO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167841
• 关键词: Archives; Arts; Blood capillaries; Charge; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Sequence; DNA Sequence Analysis; Data; Equipment; Funding; Genotype; Goals; Grant; Housing; Image; Institution; Microsatellite Repeats; Puerto Rico; Research; Research Infrastructure; Research Personnel; Resources; Source; Students; System; Technology; Training; United States National Institutes of Health; Universities; Work; capillary; cost; design

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The DNA Sequencing and Multilocus Genotyping Facility is located at the University of Puerto Rico-Rio Piedras campus (UPR-RP). The Sequencing and Genotyping Facility is designed to provide students and researchers in Puerto Rico with access to state-of-the-art equipment for collecting automated sequence and genotype data. The Facility is also charged with training students and researchers at AABRE associated institutions on how to obtain, archive, and analyze DNA sequence and multi-locus genotype data. The overarching goal of the facility is to create an alliance of researchers and students who share core research technology and who work together to strengthen scientific infrastructure and research competitiveness within Puerto Rico. The facility houses state-of-the-art equipment for automated DNA sequencing and genotyping including a MegaBace 1000 capillary DNA sequencer, Li-Cor NEN Global DNA Analyzer System and Odyssey Infrared Imaging System. The facility is open to any student or researcher at any AABRE-PR institution and provides technical support and "at-cost" DNA sequencing, SNP analysis using the SNuPe platform, and multi-locus genotyping using microsatellites and AFLPs.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 DNA测序和多焦点基因分型设备位于波多黎各Rico-Rio Piedras校园（UPR-RP）。 测序和基因分型设施旨在为波多黎各的学生和研究人员提供用于收集自动序列和基因型数据的最先进设备。 该设施还负责ABRE相关机构的培训学生和研究人员，介绍了如何获取，存档和分析DNA序列和多基因型基因型数据。 该设施的总体目标是建立一个共享核心研究技术并共同努力增强波多黎各内部科学基础设施和研究竞争力的研究人员和学生的联盟。 该设施设有用于自动DNA测序和基因分型的最先进的设备，包括1000个毛细管DNA Sequencer，Li-Cor NEN Global DNA Analyzer系统和Odyssey红外成像系统。 该设施向任何ABRE PR机构的任何学生或研究人员开放，并提供技术支持和“ AT成本” DNA测序，使用SNUPE平台分析SNP分析以及使用Microsatellites和AFLP的多洛克斯基因分型。