IMAGING CORE

成像核心

• 批准号: 8168246
• 负责人: SIDNEY Waldo WHITEHEART
• 金额: $ 1.49万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168246
• 关键词: Cells; Cellular Structures; Color; Computer Retrieval of Information on Scientific Projects Database; Computer software; Computers; Core Facility; Data Analyses; Data Storage and Retrieval; Funding; Grant; Housing; Image; Imaging Techniques; Individual; Institution; Internet; Life; Microscope; Preparation; Publications; Research; Research Personnel; Resolution; Resources; Sampling; Slide; Source; Spottings; Staging; Techniques; United States National Institutes of Health; cellular imaging; digital; digital imaging; flexibility; image processing; instrument; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The imaging core facility houses three epifluorescence microscopes (Nikon Eclipse 600, Zeiss Axiovert 100 and 200M) with digital image capture capability (Spot CE and Orca ER digital cameras with Metaview and Open Lab software). The Eclipse 600 will allow the high resolution imaging of cells and sub-cellular compartments. This instrument can be used for detailed analysis of various cellular structures. The core also contains two inverted epifluorescence microscopes. The Axiovert 200M is equipped with stage controllers to allow long term analysis of live cells. The Axiovert 100 can be used for analysis of cells in tissue culture dishes or fixed samples on slides. The flexibility of the instruments in the facility will enable the core users a wide range of analytical techniques with which to pursue their individual projects. The facility contains two computers for image processing. The computers have complete internet access and network access to color printers and to data storage. The core director, Dr. Whiteheart, will be available to offer advice on sample preparation, on data analysis, and on imaging procedures. In addition, Dr. Whiteheart will also advise in the preparation of figures and selection of images for publication.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 成像核心设施配备三台落射荧光显微镜（Nikon Eclipse 600、Zeiss Axiovert 100 和 200M），具有数字图像捕获功能（带有 Metaview 和 Open Lab 软件的 Spot CE 和 Orca ER 数码相机）。 Eclipse 600 可对细胞和亚细胞区室进行高分辨率成像。该仪器可用于各种细胞结构的详细分析。核心还包含两个倒置落射荧光显微镜。 Axiovert 200M 配备载物台控制器，可对活细胞进行长期分析。 Axiovert 100 可用于分析组织培养皿中的细胞或载玻片上的固定样品。该设施中仪器的灵活性将使核心用户能够使用广泛的分析技术来开展他们的个人项目。该设施包含两台用于图像处理的计算机。这些计算机具有完整的互联网访问权限以及对彩色打印机和数据存储的网络访问权限。核心主任 Whiteheart 博士将提供有关样品制备、数据分析和成像程序的建议。此外，Whiteheart 博士还将就数据的准备和出版图像的选择提供建议。