EFFECTS OF SPECIFIC SPERMATID-EXPRESSED Y CHROMOSOME GENES ON SPERM FUNCTION

特定精子细胞表达的 Y 染色体基因对精子功能的影响

• 批准号: 8167754
• 负责人: Monika A Ward
• 金额: $ 22.91万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167754
• 关键词: Affect; Assisted Reproductive Technology; Chromosomes; Complement; Computer Retrieval of Information on Scientific Projects Database; Failure; Fertilization; Fertilization in Vitro; Funding; Genes; Genetic; Germ Cells; Goals; Grant; Health; Human; Infertility; Institution; Intracytoplasmic Sperm Injections; Molecular Biology; Pathway interactions; Play; Research; Research Personnel; Resources; Role; Source; Spermatids; Spermatogenesis; Testing; Testis; United States National Institutes of Health; Work; Y Chromosome; arm; combat; male; men; mouse model; sex determination; sperm function; sry Genes

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Y chromosome was once thought to be responsible only for turning on sex determination towards the male pathway via the testis determinant gene Sry. However, subsequent studies revealed a number of other genes located on this chromosome that are likely to encode information important for formation and function of the male gametes. The Y chromosome was once thought to be responsible only for turning on sex determination towards the male pathway via the testis determinant gene Sry. However, subsequent studies revealed a number of other genes located on this chromosome that are likely to encode information important for formation and function of the male gametes. The goal of this project is to define the roles of certain Y encoded genes. More specifically, we seek to (1) unveil the function and mechanism of action of genes encoded on the Y chromosome long arm, (2) test whether testis determinant gene Sry plays a role in sperm function; (3) define the minimum Y gene complement that is sufficient to generate gametes capable of achieving successful fertilization. This will be done through the analysis of unique mouse models lacking various parts of the Y chromosome, and use of assisted reproduction technologies (ART): intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF), in combination with molecular biology approaches. Deletions within the male specific region of the Y chromosome are a common genetic cause of spermatogenic failure, making men carrying affected Y chromosome a target group for infertility treatment via ART. Given the known and potential problems associated with the use of ART in humans, it is essential to continue efforts on identifying the underlying causes of infertility. Our work will advance understanding of the Y chromosome encoded genes role in spermatogenesis and sperm function and ultimately may help to combat infertility, a major health problem in U.S.A.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 曾经认为，Y染色体仅是通过睾丸决定性基因sry向男性途径打开性别途径的责任。 但是，随后的研究揭示了该染色体上的许多其他基因可能对雄性配子的形成和功能很重要的信息进行编码。 曾经认为，Y染色体仅是通过睾丸决定性基因sry向男性途径打开性别途径的责任。 但是，随后的研究揭示了该染色体上的许多其他基因可能对雄性配子的形成和功能很重要的信息进行编码。 该项目的目的是定义某些Y编码基因的作用。 更具体地说，我们试图（1）揭示在Y染色体长臂上编码基因的作用和机理，（2）测试睾丸决定性基因是否在精子功能中起作用； （3）定义最低Y基因补体足以产生能够获得成功施肥的配子。 这将通过分析缺乏Y染色体各个部分的独特小鼠模型，以及使用辅助生殖技术（ART）：结合分子生物学方法结合使用辅助繁殖技术（ART）：内部胞质精子注射（ICSI）和体外受精（IVF）。 Y染色体的男性特异性区域内的缺失是精子衰竭的常见遗传原因，使携带受影响的Y染色体的男性成为通过ART进行不育治疗的目标群。 鉴于与人类使用艺术相关的已知和潜在问题，必须继续努力确定不育的根本原因。 我们的工作将促进对Y染色体编码基因在精子发生和精子功能中的作用的了解，并最终可能有助于打击不孕症，这是美国的主要健康问题。