Behavioral and Physiological Phenotyping

行为和生理表型

基本信息

批准号：
8179838
负责人：
ALEXANDER A BACHMANOV
金额：
$ 12.77万
依托单位：
MONELL CHEMICAL SENSES CENTER
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-05 至 2016-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8179838
关键词：
Anesthesia procedures Animal Model Animals Anthropology Applications Grants Area Basic Science Behavior Behavioral Biochemistry Body Weight Brain Carbon Dioxide Chemical Engineering Chemical Models Chemicals Collaborations Consultations Data Data Analyses Development Dissection Ecology Electrophysiology (science)Endocrinology Ensure Equipment Equipment and Supplies Experimental Designs Faculty Feeding behaviors Fiber Flavoring Funding Genetic Genetic Engineering Genetically Engineered Mouse Genotype Goals Grant Histology Human Resources Immunology Individual Inflammation Institutes Instruction Interdisciplinary Study Laboratories Maintenance Maps Measures Metabolic Microbiology Microscopic Microscopy Mission Molecular Biology Monitor Motor Activity National Institute on Deafness and Other Communication Disorders Nerve Neurosciences Operative Surgical Procedures Organic Chemistry Oxygen Pharmacology Phenotype Physiological Physiology Production Protocols documentation Psychophysics Public Health Research Research Personnel Resources Scientist Services Smell Perception System Taste Perception Taste Threshold Techniques Testing Tissues Training Training Support Translations Use Effectiveness Zoology base conditioning design drinking behavior experience hedonic improved instrumentation irritation multidisciplinary nutrition preference prototype repaired research study response

Anesthesia procedures Animal Model Animals Anthropology Applications Grants Area Basic Science Behavior Behavioral Biochemistry Body Weight Brain Carbon Dioxide Chemical Engineering Chemical Models Chemicals Collaborations Consultations Data Data Analyses Development Dissection Ecology Electrophysiology (science)Endocrinology Ensure Equipment Equipment and Supplies Experimental Designs Faculty Feeding behaviors Fiber Flavoring Funding Genetic Genetic Engineering Genetically Engineered Mouse Genotype Goals Grant Histology Human Resources Immunology Individual Inflammation Institutes Instruction Interdisciplinary Study Laboratories Maintenance Maps Measures Metabolic Microbiology Microscopic Microscopy Mission Molecular Biology Monitor Motor Activity National Institute on Deafness and Other Communication Disorders Nerve Neurosciences Operative Surgical Procedures Organic Chemistry Oxygen Pharmacology Phenotype Physiological Physiology Production Protocols documentation Psychophysics Public Health Research Research Personnel Resources Scientist Services Smell Perception System Taste Perception Taste Threshold Techniques Testing Tissues Training Training Support Translations Use Effectiveness Zoology base conditioning design drinking behavior experience hedonic improved instrumentation irritation multidisciplinary nutrition preference prototype repaired research study response

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项目摘要

SIGNIFICANCE. Many Monell scientists conduct studies with animals and need to characterize their chemosensory phenotypes. They will benefit from the Phenotyping Core in the following ways. 1) This Core will provide a centralized resource of equipment and supplies that will receive dedicated support and regular maintenance. The Core will also centralize labor-intensive tasks, such as making equipment for preference tests. Studies will be designed and/or conducted by Core personnel with specialized expertise in phenotyping techniques. This will be more efficient than replicating these techniques and personnel in individual laboratories. Regular use and service will ensure that equipment is always operational and available for experimentation. 2) The Core will provide access to equipment and facilities (e.g., LabMaster, surgical facility) that are not practical to maintain in individual laboratories. The Core will offer a range of services and experimental designs that are not available in individual laboratories. 3) Scientists with no experience in animal phenotyping will receive training and will have access to equipment and expertise of the Core. This will facilitate their research and help them to collect preliminary data for grant applications. 4) This Research Core will be an integral component of the Core Center. Interactions between the Phenotyping Core and other Research Cores will facilitate collaborative and interdisciplinary studies that are often not feasible for individual laboratories. For example, the Phenotyping and Histology Cores will be used to characterize genetically engineered mice generated from constructs produced using the Molecular Biology Core. In forward genetics studies, the same animals will be examined using the Phenotyping and Genotyping Cores, and the data obtained will be used for chromosomal mapping studies. We estimate that 9 of the 13 R01 grants in our Research Base will use services provided by this Core at moderate to extensive levels. We also expect that 11 current faculty research groups at Monell will use the services provided by this Core. As a result, the Phenotyping Core will improve the efficiency and quality of animal model studies at the Center, accelerate existing NIDCD-funded projects, and advance translation of the results into benefits for public health.

意义。许多Monell科学家对动物进行研究，并需要表征其化学感觉表型。他们将以以下方式从表型核心中受益。 1）该核心将提供将获得专用支持和定期维护的设备和耗材资源的集中资源。核心还将集中劳动密集型的任务，例如为偏好测试制作设备。研究将由具有表型技术专业知识的核心人员设计和/或进行。这将比复制各个实验室中的这些技术和人员更有效。定期使用和服务将确保设备始终运行并用于实验。 2）核心将提供对设备和设施（例如Labmaster，手术设施）的访问权限，这些设施在单个实验室中不可行。核心将提供各个实验室中无法使用的服务和实验设计。 3）没有动物表型经验的科学家将接受培训，并可以使用核心的设备和专业知识。这将促进他们的研究，并帮助他们收集赠款应用程序的初步数据。 4）该研究核心将是核心中心的组成部分。表型核心与其他研究核心之间的相互作用将促进对单个实验室通常不可行的协作和跨学科研究。例如，表型和组织学核心将用于表征由使用分子生物学核心产生的构建体产生的基因工程小鼠。在正向遗传学研究中，将使用表型和基因分型核心检查相同的动物，并且获得的数据将用于染色体映射研究。我们估计，我们研究基础中13个R01赠款中的9个将使用该核心提供的服务，以中等至广泛的水平。我们还预计，莫内尔（Monell）现有的11个教师研究小组将使用该核心提供的服务。结果，表型核心将提高中心动物模型研究的效率和质量，加速现有的NIDCD资助的项目，并将结果转化为公共卫生的益处。