Human Tissue & Animal Core Unit

人体组织

• 批准号: 8198069
• 负责人: John D Catravas
• 金额: $ 38.74万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-10 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8198069
• 关键词: Acute; Acute Lung Injury; Adult Respiratory Distress Syndrome; Animal Model; Animals; Biological Assay; Blood capillaries; Capillary Permeability; Cells; Dyes; Endothelial Cells; Evans blue stain; Functional disorder; Goals; Harvest; Health; Human; Instruction; Lobectomy; Lung; Measurement; Mechanics; Methods; Molecular; Mus; Permeability; Pneumonectomy; Protocols documentation; Reproducibility; Research Design; Research Personnel; Respiratory physiology; Scientist; Services; capillary; human tissue; in vivo; mortality; novel strategies; repaired; tool

Core B will have three specific aims. Specific Aim 1 is to harvest, characterize and propagate human lung microvascular endothelial cells from subjects undergoing lobectomy or pneumonectomy at MCG Health Inc.; provide said cells to investigators in all four projects for permeability, analytical and other molecular studies. Specific Aim 2 is to create and supply to all four projects mice with ALI induced by intra-tracheal instillation of gram positive or gram negative bacterial products. Specific Aim 3 is to perform in vivo lung capillary permeability (Evan's Blue Dye Method) measurements and lung function (airway mechanics) measurements for studies designed by each of the four projects.

核心 B 将有三个具体目标。 具体目标 1 是从 MCG Health Inc. 接受肺叶切除术或全肺切除术的受试者中收获、表征和繁殖人肺微血管内皮细胞；向所有四个项目的研究人员提供所述细胞，以进行渗透性、分析和其他分子研究。 具体目标 2 是创造并向所有四个项目提供由气管内诱导的 ALI 小鼠 滴注革兰氏阳性或革兰氏阴性细菌产品。 具体目标 3 是进行体内肺毛细血管通透性检测（埃文蓝染料法） 四个项目各自设计的研究的测量和肺功能（气道力学）测量。