PREPARATION AND DISTRUBUTION OF ADULT STEM CELLS

成体干细胞的制备和分配

• 批准号: 7241474
• 负责人: DARWIN Johnson PROCKOP
• 金额: $ 86.95万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7241474
• 关键词: Address; Adipocytes; Animal Disease Models; Animal Model; Antibodies; Aspirate substance; Astrocytes; Attention; Bone Marrow; Cardiomyopathies; Cell Lineage; Cells; Child; Chondrocytes; Clinical Trials; Condition; Engineering; Epitopes; Fibroblasts; Funding; Genes; Hematopoietic; Home environment; Human; Institution; Laboratories; Letters; Malignant - descriptor; Marrow; Mesenchymal Stem Cells; Methods; Muscle Cells; National Center for Research Resources; Neurons; Numbers; Osteoblasts; Osteogenesis Imperfecta; Patients; Phenotype; Population; Preparation; Procedures; Protocols documentation; Rattus; Regulation; Reporting; Research; Research Personnel; Research Project Grants; Resources; Series; Spinal cord injury; Stroke; Stromal Cells; Surface; Testing; Tissues; Viral Vector; Week; adult stem cell; cell injury; cell preparation; embryonic stem cell; gene therapy; human disease; improved; interest; research study

DESCRIPTION (provided by applicant): The overall aim of this proposal is to establish a Center for preparation, quality testing, and distribution to multiple investigators of the adult stem cells referred to as MSCs. The cells have attracted increasing attention because they are readily isolated from the patient, expanded in culture, and genetically engineered. They can differentiate into multiple cell lineages and have recently reported to have some efficacy in animal models for diseases such as osteogenesis imperfecta, spinal cord injury, stroke, and cardiomyopathies. The Center will address a critical problem that has delayed research with MSCs; the difficulties that many investigators have encountered in obtaining standardized preparations of the cells. The urgent need for a Center of the kind proposed here is reflected by the fact that 84 other investigators have provided Letters of Support for the present application. The list includes essentially all the leading investigators in this rapidly growing field. The Specific Aims are to establish a Center that will: 1) prepare a continuous supply of human MSCs that are thoroughly quality tested and distribute them on request to other investigators at multiple institutions for research on the cells; 2) prepare a similar continuous supply of rat MSCs for distribution to investigators at multiple institutions; 3) prepare MSCs from human bone marrow aspirates sent by investigators from other institutions and return the quality-tested MSCs to same investigators. Also, carry out quality testing of MSCs prepared by investigators at other institutions; and 4) develop improved methods of isolating and characterizing human MSCs using the antibodies to a series of surface epitopes that have recently been identified on the cells.

描述（由申请人提供）：该提案的总体目标是建立一个中心，用于制备、质量测试并向多名研究人员分发称为 MSC 的成体干细胞。 这些细胞引起了越来越多的关注，因为它们很容易从患者体内分离出来，在培养物中扩增，并进行基因工程改造。 它们可以分化成多种细胞谱系，最近有报道称它们在动物模型中对成骨不全、脊髓损伤、中风和心肌病等疾病有一定疗效。 该中心将解决延迟间充质干细胞研究的一个关键问题；许多研究人员在获得细胞标准化制剂时遇到的困难。 另有 84 名研究人员为本申请提供了支持信，这一事实反映了对此处提议的此类中心的迫切需要。 该名单基本上包括了这个快速发展领域的所有领先研究人员。 具体目标是建立一个中心，该中心将：1）准备持续供应经过彻底质量测试的人类间充质干细胞，并根据要求将其分发给多个机构的其他研究人员进行细胞研究； 2) 制备类似的连续供应的大鼠 MSC，以分发给多个机构的研究人员； 3）从其他机构的研究者送来的人骨髓抽吸物中制备MSC，并将经过质量检测的MSC返回给同一研究者。 另外，对其他机构的研究者制备的间充质干细胞进行质量检测； 4) 使用最近在细胞上鉴定的一系列表面表位的抗体，开发分离和表征人类 MSC 的改进方法。