INNATE IMMUNITY IN SIV INFECTION

SIV 感染的先天免疫

• 批准号: 8357512
• 负责人: Aftab A. Ansari
• 金额: $ 5.95万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357512
• 关键词: Acute; Address; Antigens; Biopsy; Blood; Cells; Colon; Disease; Funding; Genetic; Grant; HIV; Immune System Diseases; Immune response; Immunologic Deficiency Syndromes; Immunologic Monitoring; Individual; Infection; Irrigation; Macaca mulatta; Major Histocompatibility Complex Gene; Monitor; National Center for Research Resources; Natural Immunity; Natural Killer Cells; Organism; Predisposition; Primates; Principal Investigator; Research; Research Infrastructure; Resources; SIV; Small Intestines; Source; United States National Institutes of Health; Virus Replication; cost; in vivo; receptor; rectal; response; senescence

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. nfection with SIVmac251. Individuals respond very differently to infection with HIV leading to highly variable courses of disease post infection. This proposal aims to investigate a mechanism of natural immunosurveillance, termed "natural killer cells" (NK) that besides monitoring cells in the body for senescence (being too old to function properly) also sense deviation from the "normal" at the cell level. We have found that similar to the major histocompatibility complex (MHC) genes that constitute an organism's ID and dictate many of the antigen specific immune responses, innate responses also may vary due to genetic inheritance. The application will therefore focus on rhesus macaques with comparable MHC genes but distinct NK associated receptors to correlate distinct receptors with susceptibility to simian immunodeficiency (SIV) induced disease. The proposal will also address the impact of NK inhibition during acute infection on the disease course and finally whether this mechanism can be boosted by providing additional NK in vivo and whether such boost has impact on virus replication, the quality of the ensuing adaptive immune responses and disease course. All studies will investigate blood, rectal, colon and small bowel biopsies as well as broncholveolar lavages (BAL) to monitor the effect of NK before and after infection with SIVmac251.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 SIVMAC251的nFection。对感染的感染对感染的反应非常不同，导致感染后疾病的高度变化。该建议旨在研究一种天然免疫监视的机制，称为“天然杀伤细胞”（NK），除了监测体内的细胞以保持衰老（太老了，无法正常运行），还可以感觉到与细胞水平的“正常”偏离。我们发现，类似于构成生物体ID并决定许多抗原特异性免疫反应的主要组织相容性复合物（MHC）基因类似，天生反应也可能因遗传遗传而有所不同。因此，该应用将集中于具有可比的MHC基因的恒河猕猴，但与NK相关的受体不同，以将不同的受体与易感型猿猴免疫缺陷（SIV）诱导的疾病相关。该提案还将解决急性感染期间NK抑制对疾病病程的影响，最后是否可以通过在体内提供其他NK来提高这种机制，以及这种增强是否对病毒复制产生了影响，随之而来的自适应免疫反应和疾病过程的质量。所有研究都将研究血液，直肠，结肠和小肠活检以及支气管液灌注（BAL），以监测SIVMAC251感染前和之后NK的效果。