MarkI 68A Cesium-137 Gamma Irradiator

MarkI 68A 铯-137 伽马辐照器

• 批准号: 8053023
• 负责人: Wendy T Watford
• 金额: $ 37.26万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8053023
• 关键词: Ablation; Address; Age; Animals; Antigen Presentation; Antigen-Presenting Cells; Autoimmune Diseases; B-Cell Development; Biomedical Research; Bone Marrow; Cells; Cellular biology; Cesium; Chagas Disease; Communicable Diseases; Complex; Development; Environment; Funding; Funding Applicant; Goals; Growth; Immunologist; Investigation; Isotopes; Life; Maintenance; Mus; Parasites; Research; Research Personnel; T-Cell Activation; T-Lymphocyte; Transplantation; Trypanosoma cruzi; United States National Institutes of Health; Universities; Whole-Body Irradiation; biological research; design; instrument; instrumentation; irradiation; meetings; vaccine development

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Mark I Cesium 137 gamma irradiator designed for whole body irradiation of mice, a capability that is lacking at the University of Georgia. Major research applications that require this instrument include 1) ablation of host bone marrow prior to transplantation for generating bone marrow chimeric mice, 2) irradiation of antigen presenting cells for use in studies of T cell activation and development, and 3) providing an environment for efficient growth and isolation of Trypanosoma cruzi parasites. The major users group comprises 10 NIH-funded researchers from four Departments at UGA. The only irradiator currently available to UGA investigators is a Gamma Cell 200 irradiator provided through the Center for Applied Isotope Studies and, due to its age and increasing unreliability, is in need of a replacement unit that can meet the needs of the growing number of immunologists at UGA. In addition, that instrument is not appropriate for irradiating live animals for immunological research. Acquisition of the proposed irradiator will provide new capabilities that will allow investigators to address complex immunological and cell biological research problems. Specific lines of investigation that will directly benefit from the purchase of the instrument include: T and B cell development and functions in infectious and autoimmune disease settings, antigen presentation and vaccine development, and the cell biology of T. cruzi, the causative agent of Chagas' Disease. The instrumentation will therefore facilitate the realization of research goals and maintenance of NIH support for biomedical research.

描述（由申请人提供）：请求资金购买 Mark I 铯 137 伽马辐照器，该辐照器设计用于小鼠全身辐照，而佐治亚大学缺乏这种能力。需要该仪器的主要研究应用包括 1) 在移植前消融宿主骨髓以产生骨髓嵌合小鼠，2) 照射抗原呈递细胞以用于 T 细胞活化和发育的研究，以及 3) 提供环境克氏锥虫寄生虫的有效生长和分离。主要用户组由来自 UGA 四个部门的 10 名 NIH 资助的研究人员组成。目前 UGA 研究人员可用的唯一辐照器是应用同位素研究中心提供的 Gamma Cell 200 辐照器，由于其老化和越来越不可靠，需要更换装置来满足越来越多的免疫学家的需求在佐治亚大学。此外，该仪器不适用于照射活体动物进行免疫学研究。购买拟议的辐照器将提供新的功能，使研究人员能够解决复杂的免疫学和细胞生物学研究问题。购买该仪器将直接受益的具体研究领域包括：T 细胞和 B 细胞在传染性和自身免疫性疾病环境中的发育和功能、抗原呈递和疫苗开发，以及恰加斯病病原体克氏锥虫的细胞生物学' 疾病。因此，该仪器将有助于实现研究目标并维持 NIH 对生物医学研究的支持。