Acquisition of a 600 MHz NMR cryoprobe and 600 MHz spectrometer console upgrade

采购 600 MHz NMR 冷冻探头和 600 MHz 光谱仪控制台升级

• 批准号: 8050694
• 负责人: VALERIE COPIE
• 金额: $ 60万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2013-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8050694
• 关键词: Biochemical; Biochemistry; Biological; Biomedical Research; Cellular biology; Chemicals; Chemistry; Computer software; Data Collection; Development; Employment; Engineering; Frequencies; Funding; Laboratories; Microbial Biofilms; Molecular Biology; Montana; Neurosciences; Performance; Postdoctoral Fellow; Research; Research Infrastructure; Research Personnel; Sampling; Solubility; Solutions; Structural Chemistry; System; Systems Biology; Time; Training; United States National Institutes of Health; Universities; base; graduate student; instrument; instrumentation; metabolomics; programs; prospective; research study; structural biology

DESCRIPTION (provided by applicant): Support is requested to acquire a high-sensitivity 600 MHz triple resonance (1H, 13C, 15N) TCI cryoprobe and to upgrade an existing 600 MHz (1H Larmor frequency) NMR spectrometer console at Montana State University (MSU) to a more automated and high-throughput data collection platform (Bruker Avance IIITM). This instrumentation will be used by a diverse group of researchers from MSU Departments of Chemistry and Biochemistry, Veterinary Molecular Biology, Chemical and Biochemical Engineering, the Center for Biofilm Engineering, the Department of Cell Biology and Neuroscience, and the NIH Rocky Mountain Laboratory. Currently, none of the solution NMR spectrometers in the MSU Structural Biology and Chemistry NMR Facility are equipped with a cryoprobe, and the requested instrumentation and upgrade of the spectrometer console hardware will be the first of its kind on campus. The requested instrumentation upgrades will enhance sensitivity and maximize throughput of MSU 600 MHz solution NMR spectrometer. It will contribute significantly to the successful completion of challenging NMR-based structural biology projects and the expansion of NMR metabolomics research programs that are being developed on campus. The very substantially enhanced sensitivity achieved with the requested instrumentation will allow acquisition of experiments and studies on challenging biological samples, which are currently not possible because of limited sample solubility, limited sample stability, or limited instrument time availability. The console upgrade will allow automated shimming and performance optimization, and will provide much more efficient use of the recently purchased automatic sample loading system (Bruker SampleJetTM). The combination of the console upgrade and the automatic sample loading system will allow more effective 24/7 utilization of the 600 MHz NMR instrument. The cryoprobe and upgraded console will greatly facilitate the expansion of our existing NMR metabolomics capabilities (which include ChenomxTM and AMIXTM software for metabolite profiling analysis). Enhancement of NMR structural biology and metabolomics infrastructure is desired by a number of prospective users and supports the development of a new NIH-funded P20 Center for the Analysis of Cellular Mechanisms and Systems Biology. MSU's DRX 600 NMR instrument is used quite heavily, but the enhanced sensitivity and performance provided by the requested upgrades will allow this instrument to accomplish much more. The addition of the requested instrumentation will leverage significantly enhanced biomedical research capabilities for a broad group of researchers on the MSU-Bozeman campus. The requested instrumentation will also provide opportunities for new research directions in translational biomedical research, will support expanded systems biology approaches at MSU, and will contribute to the training and employment of numerous graduate students, post-doctoral fellows, and research staff. PUBLIC HEALTH RELEVANCE: We request support from the NIH to enhance the nuclear magnetic resonance (NMR) biomedical research capabilities of Montana State University. The requested instrumentation will enhance the research capabilities of a broad group of scientists involved in better understanding the molecular basis of iron homeostasis, host-pathogen interactions, viral infections, Alzheimer's, diabetes, AIDS, and other important human diseases.

描述（由申请人提供）：请求支持在蒙大拿州立大学 (MSU) 获取高灵敏度 600 MHz 三重共振（1H、13C、15N）TCI 冷冻探针并升级现有 600 MHz（1H 拉莫尔频率）NMR 波谱仪控制台）到更加自动化和高通量的数据收集平台（Bruker Avance IIITM）。该仪器将由来自密歇根州立大学化学和生物化学系、兽医分子生物学系、化学和生化工程系、生物膜工程中心、细胞生物学和神经科学系以及 NIH 落基山实验室的不同研究人员小组使用。目前，密歇根州立大学结构生物学和化学核磁共振设施中的解决方案核磁共振波谱仪都没有配备冷冻探针，而所需的仪器和波谱仪控制台硬件的升级将是校园内的第一个此类仪器。 所要求的仪器升级将提高 MSU 600 MHz 解决方案 NMR 波谱仪的灵敏度并最大限度地提高吞吐量。它将为成功完成具有挑战性的基于 NMR 的结构生物学项目以及扩展校园内正在开发的 NMR 代谢组学研究项目做出重大贡献。通过所需仪器实现的灵敏度大幅提高将允许对具有挑战性的生物样品进行实验和研究，而由于样品溶解度有限、样品稳定性有限或仪器可用时间有限，目前这是不可能的。控制台升级将允许自动匀场和性能优化，并将更有效地使用最近购买的自动样品加载系统（Bruker SampleJetTM）。控制台升级和自动样品加载系统的结合将允许 600 MHz NMR 仪器更有效地 24/7 使用。 冷冻探针和升级后的控制台将极大地促进我们现有 NMR 代谢组学功能的扩展（包括用于代谢物分析的 ChenomxTM 和 AMIXTM 软件）。许多潜在用户希望增强 NMR 结构生物学和代谢组学基础设施，并支持 NIH 资助的新 P20 细胞机制和系统生物学分析中心的开发。 MSU 的 DRX 600 NMR 仪器使用率相当高，但所要求的升级所提供的增强的灵敏度和性能将使该仪器能够完成更多工作。添加所需的仪器将显着增强密歇根州立大学博兹曼校区广大研究人员的生物医学研究能力。所需的仪器还将为转化生物医学研究的新研究方向提供机会，支持密歇根州立大学扩展的系统生物学方法，并将有助于众多研究生、博士后研究员和研究人员的培训和就业。 公共卫生相关性：我们请求 NIH 的支持，以增强蒙大拿州立大学的核磁共振 (NMR) 生物医学研究能力。所需的仪器将增强广大科学家的研究能力，以更好地了解铁稳态、宿主与病原体相互作用、病毒感染、阿尔茨海默病、糖尿病、艾滋病和其他重要人类疾病的分子基础。

项目成果

Exposure of Methicillin-Resistant Staphylococcus aureus to Low Levels of the Antibacterial THAM-3ΦG Generates a Small Colony Drug-Resistant Phenotype.

将耐甲氧西林金黄色葡萄球菌暴露于低水平的抗菌 THAM-3μG 会产生小菌落耐药表型。

• DOI: 10.1038/s41598-018-28283-3
• 发表时间: 2018
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Weaver,AlanJ;Peters,TamiR;Tripet,Brian;VanVuren,Abigail;Rakesh;Lee,RichardE;Copié,Valérie;Teintze,Martin
• 通讯作者: Teintze,Martin

Metabolomic and elemental profiling of human tissue in kidney cancer.

• DOI: 10.1007/s11306-021-01779-2
• 发表时间: 2021-03-04
• 期刊: Metabolomics : Official journal of the Metabolomic Society
• 作者: Nizioł J;Copié V;Tripet BP;Nogueira LB;Nogueira KOPC;Ossoliński K;Arendowski A;Ruman T
• 通讯作者: Ruman T