Cholinergic Circuitry in Auditory Brainstem

听觉脑干中的胆碱能回路

• 批准号: 7339302
• 负责人: Susan Diane Motts
• 金额: $ 2.5万
• 依托单位: NORTHEAST OHIO MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7339302
• 关键词: Acetylcholine; Acoustics; Address; Age; Aging; Area; Arousal; Auditory; Auditory system; Axon; Brain; Brain Stem; Cell Nucleus; Cells; Cholinergic Agents; Cholinergic Agonists; Cholinergic Receptors; Complex; Data; Disease; Dorsal; Electron Microscopy; Frequencies; Functional disorder; Immunohistochemistry; Individual; Inferior Colliculus; Knowledge; Lateral; Localized; Medial; Neuromodulator; Pathway interactions; Pedunculopontine Tegmental Nucleus; Physiological; Play; Presbycusis; Process; Property; Public Health; Range; Reporting; Role; Sensory; Sleep Wake Cycle; Source; Stimulus; Structure; Suggestion; Synapses; System; Tegmentum Mesencephali; Testing; Thalamic structure; Visual system structure; Work; auditory nuclei; auditory pathway; cholinergic; design; gamma-Aminobutyric Acid; insight; research study; response; sensory system

The inferior colliculus (1C)is a central structure in the brainstem auditory pathways and processes almost all ascending auditory sensory information. Acetylcholine is a well-studied neuromodulator and is reported to modulate the responses of cells in the 1C to acoustic stimuli. The vast majority of 1C cells respond to cholinergic agonists, and cholinergic receptors have been localized throughout the 1C. Cholinergic effects contribute to a variety of response properties, including setting a general level of sensitivity as well as altering the selectivity of a cell for properties such as stimulus frequency. There are also suggestions that cholinergic inputs can activate inhibitory circuits in the 1C, but the targets of the cholinergic axons are unknown. Overall, interpreting the results of physiological studies has been hindered by a lack of data on the cholinergic circuitry. Even the source of cholinergic input to the 1C is unknown. Characterizing this circuitry will be an important step in understanding the roles of acetylcholine in auditory function. Multilabel retrograde tracing, immunohistochemistry, and electron microscopy will be used to identify the source(s) of cholinergic input to the 1C and to characterize their collateral projections and synaptic targets Relevance to Public Health: The proposed experiments will fill a large gap in our understanding of cholinergic circuits as they relate to the 1C. A greater understanding of this circuitry will provide a framework for interpreting experiments relating to many aspects of 1C function, as well as experiments related to arousal, sleep/wake cycles, and age- related hearing loss. A better understanding of the circuitry will provide an opportunity to expand our knowledge of the function and dysfunction of the auditory system and will provide insights into the design of new experiments relating to auditory system disorders.

下丘 (1C) 是脑干听觉通路的中心结构，处理几乎所有的听觉通路。 上行听觉感觉信息。乙酰胆碱是一种经过充分研究的神经调节剂，据报道 调节 1C 细胞对声刺激的反应。绝大多数 1C 细胞对 胆碱能激动剂和胆碱能受体已定位于整个 1C。胆碱能作用 有助于各种响应属性，包括设置一般灵敏度水平以及 改变细胞对刺激频率等特性的选择性。也有建议称 胆碱能输入可以激活 1C 中的抑制回路，但胆碱能轴突的目标是 未知。总体而言，由于缺乏关于生理学研究的数据，解释生理学研究的结果受到了阻碍。 胆碱能电路。甚至 1C 的胆碱能输入来源也是未知的。表征该电路 将是理解乙酰胆碱在听觉功能中的作用的重要一步。多标签 将使用逆行追踪、免疫组织化学和电子显微镜来确定 1C 的胆碱能输入并表征其侧枝投射和突触目标 与公共卫生的相关性： 所提出的实验将填补我们对胆碱能回路理解的巨大空白，因为它们与 1C。对该电路的更好理解将为解释相关实验提供一个框架 1C 功能的许多方面，以及与觉醒、睡眠/觉醒周期和年龄相关的实验 相关的听力损失。更好地了解电路将提供扩展我们的机会 了解听觉系统的功能和功能障碍，并将提供对听觉系统设计的见解 与听觉系统疾病有关的新实验。