Rapid, Cost-Effective Nucleic Acid Testing for Active Surveillance and Molecular

用于主动监测和分子检测的快速、经济高效的核酸检测

• 批准号: 8610431
• 负责人: Ben Hindson
• 金额: $ 64.89万
• 依托单位: BIO-RAD QL, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2012-04-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8610431
• 关键词: Rapid; Cost; Effective; Nucleic; Acid

DESCRIPTION (provided by applicant): Methicillin-resistant Staphylococcus aureus (MRSA) is the most common multidrug-resistant pathogen in healthcare settings worldwide. The use of active detection and isolation (ADI) is a proven method to prevent MRSA infections that requires the detection of the MRSA-colonized patient through active surveillance testing (AST). Our long-term goal is to develop accurate tests with rapid turn-around times (<15 minutes) that could increase the efficacy of ADI and AST for MRSA prevention and control programs and molecular diagnosis across all healthcare settings. Our hypothesis is that droplet PCR will provide accurate test results with reduced analysis time, complexity and cost thereby making nucleic acid amplification tests viable near point-of- care. We propose to develop two in vitro point-of-care nucleic acid amplification tests for detecting MRSA in patient specimens. In addition to supporting development, this project would enable a mechanism and provide support for partnership between technology developers in private industry at QuantaLife Inc., an academic collaborator at Stanford University, and healthcare professionals at the University of Mississippi Medical Center (UMMC). QuantaLife scientists and engineers will develop prototype droplet PCR instrumentation and accompanying assays. UMMC has the requisite clinical expertise, specifically in the area of healthcare acquired infections, to guide test design and requirements and critically evaluate droplet PCR performance and potential impact in real-world clinical settings. The Stanford Microfluidics Laboratory is world-renowned as a leader in electrokinetic transport in microfluidic systems and has significant experience modeling and understanding physical processes at the nano- and micro-scale for biological sample preparation, and will support QuantaLife in development of automated DNA pre-processing modules. These partnerships could accelerate the translation of droplet PCR technology from the laboratory into an instrument for near-point-of- care testing thereby impacting healthcare sooner. PUBLIC HEALTH RELEVANCE: We propose to develop rapid tests for detecting the DNA of antibiotic resistant bacteria from patient samples. If implemented near the point of patient care these tests could quickly identify people who carry these bacteria and prevent subsequent infections within hospitals and clinics. This technology makes billions of copies of DNA in tiny droplets that accurately fingerprints the bacteria.

描述（由申请人提供）：耐甲氧西林金黄色葡萄球菌（MRSA）是全球医疗保健环境中最常见的多药耐药病原体。使用主动检测和隔离（ADI）是一种可预防MRSA感染的方法，该方法需要通过主动监视测试（AST）检测MRSA-ColoNical的患者。我们的长期目标是在快速的转弯时间（<15分钟）开发准确的测试，这可能会增加ADI和AST对MRSA预防和控制程序的疗效，以及在所有医疗机构中的分子诊断。我们的假设是，液滴PCR将通过缩短的分析时间，复杂性和成本提供准确的测试结果，从而使核酸扩增测试可在附近可行。我们建议开发两个体外护理核酸扩增测试，以检测患者标本中的MRSA。除了支持开发外，该项目还将为机制提供机制，并为Quantalife Inc.的技术开发人员（Stanford University的学术合作者）与密西西比大学医学中心（UMMC）的医疗保健专业人员之间的伙伴关系提供支持。量化科学家和工程师将开发原型液滴PCR仪器和随附的测定法。 UMMC具有必要的临床专业知识，特别是在医疗保健获得的感染领域，以指导测试设计和需求，并严格评估液滴PCR性能和在现实世界中临床环境中的潜在影响。斯坦福大学微流体实验室是微流体系统中电动运输领域的领导者，在生物样品制备的纳米和微尺度上具有重要的经验和理解物理过程的经验，并将支持自动化DNA预处理模块的开发中的定量生物样品。这些合作伙伴关系可以将液滴PCR技术从实验室转化为近距离护理测试的工具，从而很快影响医疗保健。 公共卫生相关性：我们建议开发快速测试，以检测患者样品中抗生素抗生素抗生素的DNA。如果在患者护理点附近实施，这些测试可能会迅速识别携带这些细菌的人并防止随后的医院和诊所内感染。这项技术在细菌准确地指纹的细小液滴中生产了数十亿份DNA。

项目成果

High-throughput droplet digital PCR system for absolute quantitation of DNA copy number.

• DOI: 10.1021/ac202028g
• 发表时间: 2011-11-15
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Hindson, Benjamin J.;Ness, Kevin D.;Masquelier, Donald A.;Belgrader, Phillip;Heredia, Nicholas J.;Makarewicz, Anthony J.;Bright, Isaac J.;Lucero, Michael Y.;Hiddessen, Amy L.;Legler, Tina C.;Kitano, Tyler K.;Hodel, Michael R.;Petersen, Jonathan F.;Wyatt, Paul W.;Steenblock, Erin R.;Shah, Pallavi H.;Bousse, Luc J.;Troup, Camille B.;Mellen, Jeffrey C.;Wittmann, Dean K.;Erndt, Nicholas G.;Cauley, Thomas H.;Koehler, Ryan T.;So, Austin P.;Dube, Simant;Rose, Klint A.;Montesclaros, Luz;Wang, Shenglong;Stumbo, David P.;Hodges, Shawn P.;Romine, Steven;Milanovich, Fred P.;White, Helen E.;Regan, John F.;Karlin-Neumann, George A.;Hindson, Christopher M.;Saxonov, Serge;Colston, Bill W.
• 通讯作者: Colston, Bill W.

Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification.

• DOI: 10.1021/ac202578x
• 发表时间: 2012-01-17
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Pinheiro, Leonardo B.;Coleman, Victoria A.;Hindson, Christopher M.;Herrmann, Jan;Hindson, Benjamin J.;Bhat, Somanath;Emslie, Kerry R.
• 通讯作者: Emslie, Kerry R.