COBRE: UID: PROJ 2: SPATIAL STRUCTURE AND ADAPTIVE EVOLUTION OF VIRUSES

COBRE：UID：项目 2：病毒的空间结构和自适应进化

• 批准号: 8167453
• 负责人: STEPHEN M. KRONE
• 金额: $ 28.85万
• 依托单位: UNIVERSITY OF IDAHO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167453
• 关键词: Address; Affect; Agar; Applications Grants; Automobile Driving; Bacteriophages; Behavior; Biological; Biological Models; Clinical; Communities; Computer Retrieval of Information on Scientific Projects Database; Data; Environment; Evolution; Experimental Models; Funding; Grant; Heterogeneity; Human; Institution; Investigation; Joints; Microbial Biofilms; Molecular; Population; Research; Research Personnel; Resources; Role; Shapes; Source; Structure; Surface; System; Time; United States National Institutes of Health; Virus; environmental change; mathematical model; microbial; microbial community; pathogen; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Most microbial communities in natural and clinical settings are spatially structured, appearing as surface-associated populations such as biofilms. This structure profoundly affects the ecological and evolutionary forces that shape the community. This is particularly important in the battle against ever-evolving human pathogens that have ecological and evolutionary time scales much shorter than human time scales. Despite this, very little is known about the specific effects of spatial heterogeneity on the adaptive evolution of microbial pathogens. Thus, a joint experimental and theoretical investigation is proposed to uncover fundamental details about the role of spatial structure in driving evolutionary and ecological dynamics of viruses. A simple experimental model system will provide a framework for addressing these issues. This experimental system will be used to study the molecular adaptive evolution of bacteriophages infecting their bacterial hosts on agar plates under changing environmental conditions. In conjunction with these experiments, new spatially explicit mathematical models (stochastic cellular automata) will be developed and fit to empirical observations with the aims of predicting the behavior of the microbial systems, uncovering the underlying biological mechanisms that are most important in the adaptive evolution of viruses, and generating new hypotheses. This project will focus on the impact of spatial structure on adaptive evolution to a single change in environment and will generate preliminary data for an independent R01 grant application that addresses adaptive evolution in the presence of fluctuating environmental conditions.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 自然和临床环境中的大多数微生物群落都是空间结构的，表现为与表面相关的种群，例如生物膜。这种结构深刻地影响着塑造群落的生态和进化力量。这在对抗不断进化的人类病原体的斗争中尤为重要，这些病原体的生态和进化时间尺度比人类时间尺度短得多。尽管如此，人们对空间异质性对微生物病原体适应性进化的具体影响知之甚少。因此，提出了联合实验和理论研究，以揭示空间结构在驱动病毒进化和生态动力学中的作用的基本细节。一个简单的实验模型系统将为解决这些问题提供一个框架。该实验系统将用于研究在变化的环境条件下感染琼脂平板上细菌宿主的噬菌体的分子适应性进化。与这些实验相结合，将开发新的空间明确的数学模型（随机细胞自动机）并适合经验观察，旨在预测微生物系统的行为，揭示在微生物适应性进化中最重要的潜在生物机制。病毒，并产生新的假设。该项目将重点研究空间结构对单一环境变化的适应性进化的影响，并将为独立的 R01 拨款申请生成初步数据，该申请解决波动环境条件下的适应性进化问题。