BIOCHEMISTRY PROTEIN CORE

生化蛋白核心

• 批准号: 8167964
• 负责人: STEPHEN G GRABER
• 金额: $ 8.76万
• 依托单位: WEST VIRGINIA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167964
• 关键词: Area; Baculoviruses; Biological Assay; Cell Culture Techniques; Cells; Computer Retrieval of Information on Scientific Projects Database; Consultations; Core Facility; Core Protein; Data Analyses; Detection; Equipment; Experimental Designs; Funding; Grant; Institution; Laboratories; Protein Analysis; Protein Biochemistry; Proteins; Research; Research Personnel; Resources; Source; System; United States National Institutes of Health; Water; Work; detector; fast protein liquid chromatography; instrumentation; protein expression; protein purification

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Protein Biochemistry Core Facility provides instrumentation and scientific support for protein expression, purification and analysis. The facility is equipped with a separate cell culture laboratory that contains all equipment required for work with Sf9 cells and baculovirus. Cold room and bench space are available for purification and analysis of proteins. The facility includes a Waters 650 Advanced Protein Purification System, Pharmacia FPLC system, preparative and analytic scale columns, UV-Vis detector, fraction collector and other assorted equipment for analysis and detection of proteins. Other major equipment includes a Biacore X100. In addition, Core staff is available for consultations on experimental design and data analysis in all areas of protein activity and interaction assay.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 蛋白质生物化学核心设施为蛋白质表达，纯化和分析提供了仪器和科学支持。 该设施配备了一个单独的细胞培养实验室，该实验室包含与SF9细胞和杆状病毒一起工作所需的所有设备。 寒冷室和长凳空间可用于纯化和分析蛋白质。 该设施包括水域650高级蛋白质纯化系统，Pharmacia FPLC系统，制备和分析量表柱，UV-VIS检测器，分数收集器和其他各种设备，用于分析和检测蛋白质。其他主要设备包括Biacore X100。 此外，在蛋白质活性和相互作用测定的所有领域的实验设计和数据分析方面，核心人员都可以咨询。