Signaling and Motility Assays

信号传导和运动测定

• 批准号: 7534111
• 负责人: ANNE R BRESNICK
• 金额: $ 10.66万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7534111
• 关键词: Animals; Behavior; Biological Assay; Biosensor; Cancer Patient; Cause of Death; Cells; Chemotaxis; Collection; Complement; Distant; Foundations; Image; Individual; Invasive; Left; Molecular Profiling; Neoplasm Metastasis; Primary Neoplasm; Proteins; Protocols documentation; Signal Transduction; Site; Stromal Cells; Technology; Time; Travel; Tumor Cell Invasion; angiogenesis; cell behavior; cell motility; cell type; in vitro Assay; in vivo; migration; neoplastic cell; tumor; two-dimensional

Core Component B, the Signaling and Motility Assays Core, will provide the expertise, protocols, analysis and interpretation of in vitro assays that examine the individual behavior, and interaction of tumor and stromal cells. The Core performs assays examining two-dimensional motile cell behavior, three-dimensional invasion, angiogenesis and transendothelial migration. These assays permit a comprehensive examination of cell behavior during chemotaxis, and provide a rapid and complete analysis of the invasive capabilities of a given cell type prior to costly and time-consuming animal studies. Moreover these in vitro assays support and complement the in vivo studies provided by the Intravital Imaging, Invasive Cell Collection and Expression Profiling Core (Core A). Core B will also act as a liaison for the use of unique protein biosensors and for assays examining invadopod formation so that the application of these technologies is uniform amongst the projects. The in vitro assays supported by Core B provide much needed information about cellular behavior and mechanism during tumor cell invasion and intravasation.The leading cause of death in cancer patients is the ability of tumor cells to leave the primary tumor, travel to distant sites within the body and form secondary tumors. The assays provided by this Core specifically examine the individual behavior and interaction of tumor and stromal cells, and will provide the foundation for understanding the mechanisms regulating tumor cell motility and invasion.

核心组件 B，即信号传导和活力检测核心，将提供专业知识、实验方案、分析 以及检查个体行为以及肿瘤和肿瘤之间相互作用的体外测定的解释 基质细胞。核心执行检测二维运动细胞行为、三维 侵袭、血管生成和跨内皮迁移。这些分析可以进行全面的检查 趋化过程中的细胞行为，并提供对细胞侵袭能力的快速、完整的分析 在进行昂贵且耗时的动物研究之前对给定的细胞类型进行研究。此外，这些体外测定支持 并补充活体成像、侵入细胞收集和 表达分析核心（核心 A）。 Core B 还将充当独特蛋白质生物传感器使用的联络人 以及检查入侵足形成的测定，以便这些技术的应用是统一的 项目之中。 Core B 支持的体外测定提供了急需的信息 肿瘤细胞侵袭和内渗过程中的细胞行为和机制。肿瘤细胞死亡的主要原因 癌症患者的肿瘤细胞有能力离开原发肿瘤，迁移到体内较远的部位 并形成继发性肿瘤。该核心提供的分析专门检查个人行为 以及肿瘤和基质细胞的相互作用，并将为理解其机制提供基础 调节肿瘤细胞的运动和侵袭。