Mapping Of Developmental Gene Expression In Mouse Embryo

小鼠胚胎发育基因表达图谱

• 批准号: 6668442
• 负责人: David Schlessinger
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6668442
• 关键词: developmental genetics; early embryonic stage; embryo /fetus; fluorescent dye /probe; fluorescent in situ hybridization; gene expression; genetic mapping; image processing; laboratory mouse

The purpose of this project is to map the locations at which specific genes are expressed in pre-implantation mouse embryos. Dr. Minoru Ko's group in LG has used expression profiling with microarrays to identify 500-1000 genes that are differentially expressed at various stages of early mouse embryo development. Initially, fluorescence in-situ hybridization (FISH) will be used to probe the locations within the embryo where these genes are expressed. Three dimensional restoration microscopy (the DeltaVision system from Applied Precision, Inc) will be employed to image these fluorescent probes. In conjunction with other fluorescent cellular markers for DNA, cytoskeletal components, and membranes, we plan to build a 3-dimensional model of the early mouse embryo in a standardized orientation, so that we can compare the location and level of gene expression from different imaging experiments. Eventually a database will be constructed to link the imaging experiments to microarray data and other mouse genomics data sources. We have recently demonstrated the applicability of DeltaVision as an imaging system for these experiments, and are currently evaluating fluorescent probe technology for use in FISH.

该项目的目的是绘制特定基因在植入前小鼠胚胎中表达的位置图。 LG 的 Minoru Ko 博士小组使用微阵列表达谱来识别在早期小鼠胚胎发育的各个阶段差异表达的 500-1000 个基因。最初，荧光原位杂交（FISH）将用于探测胚胎内这些基因表达的位置。三维修复显微镜（Applied Precision, Inc 的 DeltaVision 系统）将用于对这些荧光探针进行成像。结合其他 DNA、细胞骨架成分和膜的荧光细胞标记，我们计划以标准化方向构建早期小鼠胚胎的 3 维模型，以便我们可以比较不同成像的基因表达位置和水平实验。最终将构建一个数据库，将成像实验与微阵列数据和其他小鼠基因组数据源联系起来。我们最近展示了 DeltaVision 作为这些实验成像系统的适用性，目前正在评估用于 FISH 的荧光探针技术。