DE PEDIATRIC COBRE: CELL SCIENCE CORE

DE PEDIATRIC COBRE：细胞科学核心

• 批准号: 8360759
• 负责人: ROBERT MASON
• 金额: $ 12.88万
• 依托单位: ALFRED I. DU PONT HOSP FOR CHILDREN
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360759
• 关键词: Biological; Biotechnology; Cell Line; Cells; Cellular biology; Centers of Research Excellence; Childhood; Delaware; Development; Fibroblasts; Funding; Grant; Human; Institutes; Molecular; Molecular Biology; National Center for Research Resources; Pediatric Research; Preparation; Principal Investigator; Protein Biochemistry; Reagent; Research; Research Infrastructure; Resources; Science; Services; Source; Tissue Sample; United States National Institutes of Health; Universities; base; cost; human subject; immortalized cell; lymphoblastoid cell line; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A Cell Science Core is critical to the development of all five of the projects in this Center. The Core brings together all of the cell and molecular biological resources available within the department, and provides access to additional services available at the University of Delaware and the Delaware Biotechnology Institute. The Cell Science Core will provide services and resources for preparative and analytical studies for cell biology, protein biochemistry and molecular biology. Preparative Facility: This facility will provide a centralized staffed tissue culture facility for preparation of anonymized EBV-immoralized lymphoblastoid cell lines and fibroblast cultures from human subjects and banking of human research tissues and samples, and will also maintain other immortalized cell lines and serve as a bank for these call-based reagents. Analytical Facility: This facility will consist of the Cell Biology Resource, Protein Biochemistry Resource and Molecular Biology subcores.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 细胞科学核心对于该中心所有五个项目的发展至关重要。 该核心汇集了该部门内所有可用的细胞和分子生物资源，并提供特拉华大学和特拉华生物技术研究所提供的其他服务。 细胞科学核心将为细胞生物学、蛋白质生物化学和分子生物学的制备和分析研究提供服务和资源。 制备设施：该设施将提供一个集中配备人员的组织培养设施，用于制备来自人类受试者的匿名 EBV 永生化淋巴母细胞系和成纤维细胞培养物以及人类研究组织和样本的库，并且还将维护其他永生化细胞系并作为这些基于呼叫的试剂的银行。 分析设施：该设施将由细胞生物学资源、蛋白质生物化学资源和分子生物学子核心组成。