PROTEOMICS CORE

蛋白质组学核心

• 批准号: 8360573
• 负责人: Haining Zhu
• 金额: $ 9.56万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360573
• 关键词: Amino Acid Sequence; Centers of Research Excellence; Chemicals; Computer software; Data Analyses; Detection; Diamond; Distant; Dyes; Funding; Gel; Grant; High Pressure Liquid Chromatography; Individual; Internet; Laboratories; Licensing; Mass Spectrum Analysis; Molecular; National Center for Research Resources; Peptide Mapping; Peptide Sequence Determination; Post-Translational Protein Processing; Postdoctoral Fellow; Preparation; Principal Investigator; Pro-Q aerosol foam; Proteins; Proteomics; Research; Research Infrastructure; Research Personnel; Resources; Robotics; Sampling; Sequence Analysis; Services; Site; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staining method; Stains; Students; System; Training; United States National Institutes of Health; Water; Work; aerobic respiration control protein; base; cost; data acquisition; design; human disease; mass spectrometer; protein profiling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The proteomics core is designed to provide quantitative and qualitative protein profiling and identification analysis, as well as post-translational modification and sequence analysis through a combination of 2D gel, mass spectrometry and Edman sequencing analysis services. The core is equipped with Bio-Rad IPG and SDS-PAGE apparatus for 2D gel analysis, a Typhoon phosphorimager/scanner system for detection and quantitation of fluorescent protein dyes (Cy-dyes) and stains (SYPRO-Ruby, Pro-Q Diamond) used to analyze 1D and 2D gels, a Ciphergen SELDI-TOF protein profiling system, an ABI QSTAR II Q-TOF Mass Spectrometer with both MALDI and LC/ESI source capabilities, Waters HPLC systems and multiple PCs for data acquisition and analysis including a site license for PDQuest software and a 5 station site license for Ciphergen software. Fully trained technical staff will assist investigators with the full range of proteomics analytical services. The staff will instruct investigators and their laboratory staff (students, post-doctoral fellows and technicians) in sample preparation, 2D gel analysis and quantitation by fluorescent staining methods. Protein fragmentation, mass peptide mapping and protein sequencing by both MS/MS mass spectrometry and chemical sequencing will be performed by Dr. Beach on samples provided by individual investigators. Preparation and analysis of samples with the Ciphergen system will be facilitated by automated robotic work stations. Data analysis will be performed by investigators and their colleagues through multiple distant work stations connected to the Ciphergen system via the web.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 蛋白质组学核心旨在通过2D凝胶，质谱和Edman测序分析服务的组合提供定量和定性的蛋白质分析和鉴定分析以及翻译后修饰和序列分析。该核心配备了生物RAD IPG和SDS-PAGE设备，可用于2D凝胶分析，一种台风磷光剂/扫描仪系统，用于检测和定量荧光蛋白质染料（cy-dyes）（cy-dyes）和污渍（Sypro-Ruby，Pro-Q diamond）用于分析1D和2D凝胶，一个Ciphergen Seldi-TOF蛋白谱系统，ABI QSTAR II QSTAR II Q-TOF质谱仪，具有MALDI和LC/ESI源功能，Waters HPLC系统和多个用于数据获取和分析的PC，包括站点PDQuest软件的许可证和Ciphergen软件的5个站点许可证。训练有素的技术人员将协助调查人员提供各种蛋白质组学分析服务。工作人员将指导调查人员及其实验室工作人员（学生，博士后研究员和技术人员）进行样品制备，2D凝胶分析和荧光染色方法的定量。 Beach博士将对单个研究人员提供的样品进行蛋白质碎片化，质量肽图和蛋白质测序。自动机器人工作站将促进使用Ciphergen系统对样品进行准备和分析。数据分析将由研究人员及其同事通过通过网络连接到Ciphergen系统的多个遥远的工作站进行。