Pressure-jump apparatus for time-resolved and static X-ray diffraction studies of conformational/phase transitions & structure in soft matter systems

用于构象/相变的时间分辨和静态 X 射线衍射研究的压力跳跃装置

• 批准号: ST/F001401/1
• 负责人: John Seddon
• 金额: $ 43.78万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=ST%2FF001401%2F1
• 关键词: Pressure; jump; apparatus; time; resolved

There is currently a pressing need for the development of techniques that will allow experimentalists to probe the time-dependent structural behaviour of out-of-equilibrium soft condensed matter systems. An ideal technique for monitoring and triggering the structural evolution of such transformations within the millisecond timescale is time-resolved X-ray scattering, employing pressure as the triggering variable. The p-jump technique can be applied quite generally to the study of soft condensed matter and biomolecular phase transformations, such as lyotropic liquid crystal phase transitions, micelle-vesicle transformations, DNA unwinding, and protein folding. To this end we aim to develop and construct a millisecond pressure-jump X-ray cell for use at the Non-Crystalline Scattering beamline I22 at Diamond. We will also develop user-friendly apparatus control, integrated with the beamline software currently under development for I22. Although this apparatus is primarily designed for pressure-jump experiments, it can also be used for rapid scanning of pressure in the range 1 bar - 5 kbar, allowing pressure-temperature phase diagrams to be determined rather efficiently. It should also be noted that the cell will be capable of holding a fixed pressure for specific experiments.

目前迫切需要开发技术，使实验学家能够探测非平衡软凝聚态物质系统的时间依赖性结构行为。在毫秒时间尺度内监测和触发此类转变的结构演化的理想技术是时间分辨 X 射线散射，采用压力作为触发变量。 p-jump技术可以广泛应用于软凝聚态物质和生物分子相变的研究，例如溶致液晶相变、胶束-囊泡转变、DNA解旋和蛋白质折叠。为此，我们的目标是开发和构建毫秒压跃 X 射线单元，用于 Diamond 的非晶体散射光束线 I22。我们还将开发用户友好的设备控制，与目前正在开发的 I22 光束线软件集成。虽然该装置主要设计用于压力跃变实验，但它也可用于快速扫描 1 bar - 5 kbar 范围内的压力，从而可以相当有效地确定压力-温度相图。还应该注意的是，该单元将能够为特定实验保持固定压力。

项目成果

A 3-D hexagonal inverse micellar lyotropic phase.

3-D 六方反胶束溶致相。

• DOI: 10.1021/ja809280r
• 发表时间: 2009-02-11
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: G. Shearman;A. Tyler;N. Brooks;R. Templer;O. Ces;R. V. Law;J. Seddon
• 通讯作者: J. Seddon

A pressure-jump time-resolved X-ray diffraction study of cubic-cubic transition kinetics in monoolein.

单油酸酯立方-立方转变动力学的压力跳跃时间分辨 X 射线衍射研究。

• DOI: 10.1021/la7023378
• 发表时间: 2008-03-18
• 期刊: Langmuir : the ACS journal of surfaces and colloids
• 作者: C. Conn;O. Ces;A. Squires;X. Mulet;R. Winter;S. Finet;R. Templer;J. Seddon
• 通讯作者: J. Seddon

Ordered micellar and inverse micellar lyotropic phases

有序胶束和反胶束溶致相

• DOI: 10.1080/02678292.2010.484917
• 发表时间: 2010-07-05
• 期刊: Liquid Crystals
• 影响因子: 2.2
• 作者: G. Shearman;A. Tyler;N. Brooks;R. Templer;O. Ces;Robert V. Law;J. Seddon
• 通讯作者: J. Seddon

Automated high pressure cell for pressure jump x-ray diffraction.

用于压力跳跃 X 射线衍射的自动化高压单元。

• DOI: http://dx.10.1063/1.3449332
• 发表时间: 2010
• 期刊: The Review of scientific instruments
• 作者: Brooks NJ

Quantitative model for the kinetics of lyotropic phase transitions involving changes in monolayer curvature

涉及单层曲率变化的溶致相变动力学的定量模型

• DOI: http://dx.10.1039/b911762h
• 发表时间: 2009
• 期刊: Soft Matter
• 影响因子: 3.4
• 作者: Squires A