SPIDER TRAINING AND DISSEMINATION

蜘蛛训练和传播

• 批准号: 8172280
• 负责人: Ardean Leith
• 金额: $ 2.16万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-07 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172280
• 关键词: Biological; Classification; Computer Retrieval of Information on Scientific Projects Database; Computer software; Data; Data Analyses; Documentation; Electron Microscopy; Electrons; Funding; Grant; Image; Institution; Internet; Java; Nature; Paper; Procedures; Protocols documentation; Research; Research Personnel; Resources; Running; Sampling; Source; Spiders; Training; United States National Institutes of Health; Work; abstracting; base; cluster computing; design; electron tomography; image processing; macromolecule; open source; particle; reconstruction; tool; web site

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: SPIDER is an extensive image-processing software package specifically designed for 3D reconstruction in electron microscopy. It has been evolving in Dr. Frank's Lab since the late 1970's and contains appropriate tools for single particle reconstruction, classification analysis, and electron tomography, and segmentation of volumes. The current distribution consists of: + SPIDER Fast command line and procedural computation engine + Web Point-and-click GUI for image interaction + JWeb Point-and-click Java-based GUI for image interaction + SPIRE Point-and-click GUI for running SPIDER procedures + PubSub Scripted mechanism for distributing work on a compute cluster Since released as open source in 2005 there have been more than 1500 downloads of the software by recognizable registered users. SPIDER's web site is: http://www.wadsworth.org/spider_doc/spider/docs/spider.html. Documentation for particle verification for single-particle, reference-based reconstruction using multivariate data analysis and classification is provided in the following paper: + Shaikh, T.R., Trujillo, R., LeBarron, J.S., Baxter, W.T. and Frank, J. (2008) Particle-verification for single-particle, reference-based reconstruction using multivariate data analysis and classification. J. Struct. Biol. 164: 41-48. PMCID 2577219. Use of SPIRE, with sample configuration files for typical single-particle procedures, is set out in the following paper, the data for which is included in the current SPIRE distribution: + Shaikh, T., Gao, H., Baxter, W., Asturias, F., Boisset, N., Leith, A. and Frank, J. (2008) SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs. Nature Protocols 3:1941-74. PMCID 2737740.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 抽象的： 蜘蛛是一个专门设计用于电子显微镜中3D重建的大量图像处理软件包。 自1970年代后期以来，它一直在弗兰克博士的实验室中发展，并包含用于单个粒子重建，分类分析和电子断层扫描以及体积分割的合适工具。 当前的分布包括： +蜘蛛快速命令行和程序计算引擎 +用于图像互动的网络点击GUI + jweb点击点击基于Java的GUI用于图像互动 +用于运行蜘蛛手术的尖峰点击GUI +用于在计算集群上分发工作的PubSub脚本脚本机制 自2005年以开源而发布以来，已有可识别的注册用户下载了1500多个软件。 蜘蛛的网站是：http：//www.wadsworth.org/spider_doc/spider/docs/spider.html。 以下文章提供了用于使用多元数据分析和分类的单粒子粒子验证的文档：基于参考的重建： + Shaikh，T.R.，Trujillo，R.，Lebarron，J.S。，Baxter，W.T。和Frank，J。（2008）使用多元数据分析和分类的单粒子粒子验证，基于参考的重建。 J. struct。生物。 164：41-48。 PMCID 2577219。 使用Spire，并在以下论文中列出了典型的单粒子过程的样本配置文件，其中包含在当前尖峰分布中的数据： + Shaikh，T.，Gao，H.，Baxter，W.，Asturias，F.，Boisset，N.，Leith，A。和Frank，J。（2008）蜘蛛图像处理，用于单粒子重建电子显微镜的生物大分子。自然协议3：1941-74。 PMCID 2737740。