CORE--Histology

核心--组织学

• 批准号: 8076244
• 负责人: ANDREW J CONNOLLY
• 金额: $ 12.46万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8076244
• 关键词: Aorta; Aortic Aneurysm; Cardiovascular system; Cellular Morphology; Data Base Management; Dissection; Documentation; Electron Microscopy; Equipment; Frozen Sections; Histology; Histopathology; Image Analysis; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Lasers; Liquid substance; Methods; Morphology; Nitrogen; Paraffin Embedding; Pathology; Plasma; Reagent; Research; Research Personnel; Research Project Grants; Sampling; Services; Staining method; Stains; Techniques; Tissue Banking; Tissue Banks; Tissues; Transmission Electron Microscopy; base; laser capture microdissection; post-doctoral training; protein expression

The Histology Core will provide comprehensive service to the Research Projects by making available: comprehensive tissue banking; proven histology methods; reliable reagents; necessary equipment; technical assistance, and expert pathology interpretation. The Histology Core will bring not only efficiency in routine methods, but also will allow more advanced morphologic techniques to be utilized as needed. It will offer routine frozen section and paraffin-embedded histology, special histochemical stains, immunohistochemistry, immunofluorescence, photomicroscopy, image analysis, transmission electron microscopy, and laser capture microdissection. Nearly all of the necessary equipment is currently available in the Department of Pathology. These techniques will be supervised by Dr. Connolly and performed with the assistance of a Histology research assistant. This should allow investigators to assess the morphology, cellular constituents and protein expression with aortic aneurysms. The Histology Core will serve as a comprehensive Tissue Bank for this SCCOR, including database management and liquid nitrogen storage of aorta, plasma, serum, and buffy coat samples from the Projects.

组织学核心将通过以下方式为研究项目提供全面的服务： 综合组织库；经过验证的组织学方法；可靠的试剂；必要的设备； 技术援助和专家病理解释。组织学核心不仅会带来 常规方法的效率，而且还允许利用更先进的形态学技术 根据需要。它将提供常规冰冻切片和石蜡包埋组织学、特殊组织化学 染色、免疫组织化学、免疫荧光、显微术、图像分析、传输 电子显微镜和激光捕获显微切割。几乎所有必要的设备都有 目前在病理科提供。这些技术将由博士监督。 康诺利并在组织学研究助理的协助下进行。这应该允许 研究人员评估主动脉的形态、细胞成分和蛋白质表达 动脉瘤。组织学核心将作为本次 SCCOR 的综合组织库， 包括主动脉、血浆、血清和血沉棕黄层的数据库管理和液氮存储 来自项目的样本。