Neurophysiology of DBS

DBS的神经生理学

基本信息

批准号：
8076855
负责人：
Barry W Connors
金额：
$ 19.32万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-01 至 2014-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8076855
关键词：
Action Potentials Affect Affective Area Axon Basal Ganglia Behavior Behavioral Biological Neural Networks Brain Cells Chimeric Proteins Chronic Disease Electric Stimulation Excitatory Synapse FOS gene Face Frequencies Functional disorder Goals Green Fluorescent Proteins In Vitro Inhibitory Synapse Interneurons Long-Term Depression Long-Term Potentiation Maps Measurement Measures Methods Mouse Strains Mus Neocortex Neurobiology Neurons Obsessive-Compulsive Disorder Pattern Physiological Population Prefrontal Cortex Preparation Process Property Pyramidal Cells Refractory Resolution Simulate Site Slice Structure Synapses Synaptic Transmission Synaptic plasticity Testing Thalamic structure Time Training Transgenic Mice Whole-Cell Recordings addiction base conditioning extracellular hippocampal pyramidal neuron in vivo insight neocortical neuronal cell body neurophysiology neurotransmitter release postsynaptic presynaptic receptor research study response white matter

项目摘要

Project 6 will focus on the most basic neurobiological effects of DBS, i.e. how does long-lasting, high- frequency electrical stimulation of subcortical white matter affect the axons, synapses, and neurons of prefrontal regions of neocortex? To this end, we will use contemporary methods of in vitro cellular neurophysiology to make high resolution measurements from groups of specific, genetically identified cortical neurons and their synaptic connections. Several lines of GFP-expressing mice will be used. Experiments will focus on the influence of DBS on conduction properties of afferent and efferent axons, and identified types of excitatory and inhibitory synaptic connections and their local cortical circuits. We will also use a c- fos-GFP mouse line to ask how high frequency stimulation in vivo alters the physiological properties of c-fos- expressing neurons. The results of this project should illuminate the basic mechanisms of DBS in brain structures relevant to OCD. RELEVANCE (See instmctions): Obsessive Compulsive Disorder (OCD) is a chronic psychiatric illness that affects 2-3% of the worldwide population. This is disease is in the top ten dehabilitating diseases. This study will examine the neural network and mechanisms that underiie behaviors associated with OCD. These behaviors not limited to OCD, but are associated with a range of affective and addictive disorders. The collective proposed studies will generate new hypotheses of how dysfunctions within these brain networks are expressed across diseases and provide insight into the mechanisms underlying normal behavioral responses. PROJECT/

项目6将重点关注DBS最基本的神经生物学效应，即持久，高级皮质下白质的频率电刺激会影响轴突，突触和神经元的神经元新皮层的前额叶区域？为此，我们将使用当代体外细胞的方法神经生理学以从特定的，遗传性的皮质组中进行高分辨率测量神经元及其突触连接。将使用几行表达GFP的小鼠。实验将侧重于DBS对传入和传出轴突的传导性能的影响，并确定兴奋性和抑制性突触连接的类型及其局部皮质回路。我们还将使用C- FOS-GFP小鼠系询问体内高频刺激如何改变C-FOS-的生理特性表达神经元。该项目的结果应阐明大脑中DBS的基本机制与强迫症相关的结构。相关性（请参阅Instmctions）：强迫症（OCD）是一种慢性精神病，影响全球2-3％人口。这是疾病中的十大疾病。这项研究将检查神经与强迫症相关的行为的网络和机制。这些行为不限于强迫症，但与一系列情感和成瘾性疾病有关。集体提出的研究将产生新的假设，即这些大脑网络中的功能障碍是如何表达的疾病并提供对正常行为反应基础机制的见解。项目/