CONFOCAL MICROSCOPY AND IMAGE ANALYSIS CORE

共焦显微镜和图像分析核心

• 批准号: 8172959
• 负责人: Xavier Alvarez-Hernandez
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172959
• 关键词: Arts; Cells; Color; Computer Retrieval of Information on Scientific Projects Database; Computer software; Confocal Microscopy; Data; Data Analyses; Detection; Dimensions; Fluorochrome; Funding; Grant; Hour; Human Resources; Image; Image Analysis; Institution; Label; Lasers; Length; Life; Light; Microscope; Microscopy; Preparation; Publications; Research; Research Personnel; Resolution; Resources; Running; Scanning; Scientist; Services; Source; Spottings; System; Time; Training; United States National Institutes of Health; Width; base; in vivo

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core provides state-of-the-art confocal microscopy, multilabel fluorescent labeling and detection, and image analysis support to every Division in the TNPRC, and to numerous affiliate research scientists at several institutions. The Core has a Leica TCS SP2 laser scanning confocal microscope system equipped with 3 lasers, with 6 laser lines available, capable of simultaneously collecting information in four channels (3 fluorescent and one for differential interference contrast). The system is attached to two microscopes an upright (DMRE) and an inverted (DMIRE2), that allow for confocal microscopy of fixed preparations and also living cells. We have a separate workstation to run the Leica software for the analysis of the data collected. We also have Volocity Software for the rendition of the data in 3 dimensions. The Image analysis system for transmitted light is based in the Leica DMRE microscope, a Spot camera and the latest ImagePro software in a dedicated workstation for investigators use in image analysis. The confocal system offers such benefits as: a) Multi-dimensional imaging, as it is possible to obtain images in four dimensions, length (x axis), width (y axis), depth (z axis) and time (t). b) Resolution improvement. c) Contrast improvement. d) Multicolor imaging. Several fluorochromes can be imaged at the same time. In the last year four new users were trained and approved to use the confocal microscope without assistance. In addition, core personnel assisted other users in 180 timeslots for 750 hours. The core performed 300 imaging services, including in vivo imaging and 2 or 3 color microscopy that generated more than 400 gigabytes of data. The core directly contributed to 4 new publications.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该核心为TNPRC的每个部门以及多个机构的众多会员研究科学家提供了最先进的共聚焦显微镜，多标签荧光标签和检测以及图像分析支持。 该核心具有LEICA TCS SP2激光扫描共聚焦显微镜系统，配备3个激光器，具有6个激光线，能够同时在四个通道中收集信息（3个荧光灯（3个荧光对比度））。 该系统连接到两个直立（DMRE）和一个倒置（DMIRE2）上，该显微镜可以进行固定制剂和活细胞的共聚焦显微镜。我们有一个单独的工作站来运行Leica软件，以分析收集的数据。我们还拥有3个维度的数据的Volocity软件。用于传输光的图像分析系统基于Leica DMRE显微镜，点摄像头和最新的ImagePro软件，用于研究人员在图像分析中使用的专用工作站中。共聚焦系统提供了：a）多维成像，因为可以在四个维度，长度（x轴），宽度（y轴），深度（z轴）和时间（t）中获得图像。 b）解决方案的改进。 c）对比度改进。 D）多色成像。 可以同时成像几个荧光染色。 在过去的一年中，四个新用户接受了培训和批准，可以在没有帮助的情况下使用共聚焦显微镜。 此外，核心人员在180个时隙中协助其他用户750小时。 核心执行了300个成像服务，包括体内成像和2或3个颜色显微镜，产生了超过400 GB的数据。 核心直接为4个新出版物做出了贡献。