ALCOHOL AND TB IN NONHUMAN PRIMATES

非人类灵长类动物中的酒精和结核病

• 批准号: 8173051
• 负责人: Carol A. Mason
• 金额: $ 3.94万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173051
• 关键词: Affect; Alcohol consumption; Alcoholism; Alcohols; Animals; Cell Count; Communicable Diseases; Computer Retrieval of Information on Scientific Projects Database; Containment; Disease Outcome; Effector Cell; Funding; Gene Expression; Grant; Human; Immune response; Infection; Institution; Lung; Macaca; Macaca mulatta; Modeling; Mycobacterium tuberculosis; Research; Research Personnel; Resources; Source; Syndrome; United States National Institutes of Health; Virulent; abstracting; aerosolized; alcohol research; binge drinking; medical schools; mycobacterial; nonhuman primate; problem drinker; transcriptomics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Abstract: Mycobacterium tuberculosis (Mtb) infects two billion people worldwide. Alcoholics have a greater burden of TB than normal hosts, and alcohol worsens the progression and the outcome of the disease. Nonhuman primates best recapitulate the human TB syndrome, and Dr. Kaushal's group at the TNPRC has generated a model of human TB in macaques exposed to aerosolized Mtb [Dutta et al, J. Infect Dis, 2010, 201:]. Further, the Comprehensive Alcohol Research Center (CARC) at the LSU School of Medicine, New Orleans has considerable expertise in studying co-incident infectious diseases in a nonhuman primate model of binge alcoholism. We hypothesize that binge alcohol consumption by rhesus macaques will adversely affect the containment of virulent Mtb via alterations in the lung CD4+ Th1 effector cell number and function, causing accelerated progression of infection. We propose the following specific aims: 1. To evaluate the mycobacterial clearance of pulmonary infection with virulent Mtb in alcohol consuming rhesus monkeys. 2. Characterize the immune response in these animals as well as gene expression through transcriptomics. This project has been funded, and is expected to start as soon as appropriate BSL-3 space is available.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 摘要：结核分枝杆菌（Mtb）感染全球 20 亿人。酗酒者比正常宿主承受更大的结核病负担，并且酒精会恶化疾病的进展和结果。非人类灵长类动物最能概括人类结核病综合症，TNPRC 的 Kaushal 博士小组已经在接触雾化结核分枝杆菌的猕猴中建立了人类结核病模型 [Dutta 等人，J. Infect Dis, 2010, 201:]。此外，新奥尔良路易斯安那州立大学医学院的综合酒精研究中心 (CARC) 在研究非人类灵长类酗酒模型中的并发传染病方面拥有丰富的专业知识。 我们假设恒河猴的暴饮暴食会通过改变肺部 CD4+ Th1 效应细胞的数量和功能，对有毒力 Mtb 的遏制产生不利影响，从而导致感染加速进展。我们提出以下具体目标： 1. 评估饮酒恒河猴体内强毒结核分枝杆菌肺部感染的分枝杆菌清除情况。 2. 通过转录组学表征这些动物的免疫反应以及基因表达。 该项目已获得资助，预计在有适当的 BSL-3 空间可用后立即启动。