THE MUCOSAL DENDRITIC CELL-T CELL MILIEU AND SIV SPREAD

粘膜树突状细胞 T 细胞环境和 SIV 传播

• 批准号: 8172970
• 负责人: Melissa J Robbiani
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172970
• 关键词: Affect; Animals; Antibodies; Cells; Computer Retrieval of Information on Scientific Projects Database; Dendritic Cells; Epithelium; Funding; Grant; Hour; Immune; Infection; Institution; Ligation; Macaca; Memory; Myelogenous; Plasma; Poly I-C; Production; Regulatory T-Lymphocyte; Research; Research Personnel; Resources; SIV; Source; T-Lymphocyte Subsets; Testing; Time; United States National Institutes of Health; Virus; chemokine; cytokine; prevent; rectal; transmission process; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the first study of poly(IC) and rectal SIV transmission, we asked whether TLR ligation by poly(IC) could prevent SIV transmission or modify the course of infection in animals that were not protected. Poly(IC) was applied to the rectal epithelium of macaques either coincident with (n=7) or 24 hours prior to (n=7) SIVwt challenge. As controls, we challenged 4 animals with SIVwt without receiving poly(IC) treatment and included the 4 animals used to test the infectivity of the virus stock (n=8 total). It does not appear that either the coincident or the 24 h pre-treatment with poly(IC) affected the infection rate (coincident: 4 of 7 infected, 57%; 24 h pre: 4 of 7 infected, 57%, control: 6 of 8 infected, 75%). However, we are continuing to evaluate differences in plasma virus RNA over time (at peak and set point) as well as innate and adaptive immune parameters (memory and na¿ve T cell subsets, regulatory T cells, antibodies, DCs and the ratio of myeloid and plasmacytoid DCs, and cytokine and chemokine production) in these 18 animals.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以出现在其他 CRISP 条目中 列出的机构是。 对于中心来说，它不一定是研究者的机构。 在第一项关于 Poly(IC) 和直肠 SIV 传播的研究中，我们询问 Poly(IC) 的 TLR 连接是否可以预防 SIV 传播或改变未将 Poly(IC) 应用于直肠的动物的感染过程。猕猴的上皮细胞与（n = 7）或在（n = 7）SIVwt攻击前24小时作为对照，我们用SIVwt攻击4只动物，而不接受聚（IC）治疗，并包括使用 4 只动物测试病毒原液的感染性（总共 8 只），同时使用聚（IC）或 24 小时预处理似乎不会影响感染率（同时：7 只中有 4 只感染， 57%；24 小时前：7 名感染者中的 4 名，57%，对照：8 名感染者中的 6 名，75% 然而，我们正在继续评估血浆病毒 RNA 随时间的变化（峰值和设定值）。点）以及这 18 只动物的先天和适应性免疫参数（记忆和幼稚 T 细胞亚群、调节性 T 细胞、抗体、DC 以及髓样和浆细胞样 DC 的比例，以及细胞因子和趋化因子的产生）。