THE MUCOSAL DENDRITIC CELL-T CELL MILIEU AND SIV SPREAD

粘膜树突状细胞 T 细胞环境和 SIV 传播

• 批准号: 8172970
• 负责人: Melissa J Robbiani
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172970
• 关键词: Affect; Animals; Antibodies; Cells; Computer Retrieval of Information on Scientific Projects Database; Dendritic Cells; Epithelium; Funding; Grant; Hour; Immune; Infection; Institution; Ligation; Macaca; Memory; Myelogenous; Plasma; Poly I-C; Production; Regulatory T-Lymphocyte; Research; Research Personnel; Resources; SIV; Source; T-Lymphocyte Subsets; Testing; Time; United States National Institutes of Health; Virus; chemokine; cytokine; prevent; rectal; transmission process; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the first study of poly(IC) and rectal SIV transmission, we asked whether TLR ligation by poly(IC) could prevent SIV transmission or modify the course of infection in animals that were not protected. Poly(IC) was applied to the rectal epithelium of macaques either coincident with (n=7) or 24 hours prior to (n=7) SIVwt challenge. As controls, we challenged 4 animals with SIVwt without receiving poly(IC) treatment and included the 4 animals used to test the infectivity of the virus stock (n=8 total). It does not appear that either the coincident or the 24 h pre-treatment with poly(IC) affected the infection rate (coincident: 4 of 7 infected, 57%; 24 h pre: 4 of 7 infected, 57%, control: 6 of 8 infected, 75%). However, we are continuing to evaluate differences in plasma virus RNA over time (at peak and set point) as well as innate and adaptive immune parameters (memory and na¿ve T cell subsets, regulatory T cells, antibodies, DCs and the ratio of myeloid and plasmacytoid DCs, and cytokine and chemokine production) in these 18 animals.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 在对聚（IC）和直肠SIV传播的首次研究中，我们询问Poly（IC）通过TLR连接是否可以防止SIV传播或改变不受保护的动物的感染过程。将poly（ic）应用于猕猴的直肠上皮与（n = 7）或（n = 7）SIVWT挑战之前的（n = 7）或24小时。作为对照，我们对4只动物进行了SIVWT挑战，而无需接受聚（IC）治疗，并包括用于测试病毒库存感染的4只动物（n = 8个）。看来，与poly（IC）的重合或24小时预处理影响感染率（重合：4中的4个感染，57％； 24小时； 24 h Pre：4 of 7受感染，57％，对照：6中的6个中的8个感染，75％）。但是，我们将继续评估血浆病毒RNA随着时间的推移（在峰值和设定点）的差异，以及先天和适应性的免疫参数（记忆和NA¿