The Role of Separase During Cytokinesis in C elegans

分离酶在秀丽隐杆线虫细胞分裂过程中的作用

• 批准号: 7578442
• 负责人: Joshua Nathaniel Bembenek
• 金额: $ 2.52万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2008-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7578442
• 关键词: Affect; Alleles; Anaphase; Animals; Behavior; Biological; Caenorhabditis elegans; Cell Division Process; Cell physiology; Cells; Chromosome Segregation; Chromosomes; Condition; Cytokinesis; Cytoplasmic Structures; Defect; Embryo; Endopeptidases; Ensure; Failure; Fright; Genetic; Genetic Materials; Genetic Nondisjunction; Homologous Gene; Laboratories; Learning; Localized; Mechanics; Meiosis; Mitotic; Molecular; Osmotic Pressure; Pathway interactions; Peptide Hydrolases; Range; Role; Saccharomycetales; Signal Pathway; Sister Chromatid; Structure; Techniques; Temperature; Time; cohesion; mutant; separase; transmission process

The process of cell division requires the correct sequence and timing of various cellular processes to ensure faithful transmission of the genetic material and cellular contents. The entry to anaphase occurs upon the cleavage of sister-chromatid cohesion by a protease known as separase. Separase has recently been shown to promote anaphase spindle dynamics and cytokinesis concomitantly with chromosome segregation in budding yeast. Previous studies from our laboratory identified a temperature-sensitive allele of the separase homologue in C. elegans. Embryos from these separase-mutant worms shifted to the restrictive temperature display several meiotic and mitotic defects such as chromosome nondisjunction, osmotic and mechanical sensitivity, and cytokinesis failure. Using various genetic and cell biological techniques, I propose to uncover the mechanisms by which separase contributes to the completion of cytokinesis in the early embryo of C. elegans. Through this analysis, I hope to extend the understanding of the molecular mechanisms utilized for the temporal coordination of chromosome segregation with the onset of anaphase and cytokinesis.

细胞分裂的过程需要各种细胞过程的正确顺序和计时，以确保 遗传物质和细胞内容物的忠实传递。进入后期发生在 通过称为分离酶的蛋白酶切割姐妹染色单体的内聚力。分离酶最近被 显示可促进后期纺锤体动力学和胞质分裂并伴随染色体分离 在芽殖酵母中。我们实验室之前的研究发现了一个对温度敏感的等位基因 秀丽隐杆线虫中的分离酶同源物。这些分离酶突变蠕虫的胚胎转移到限制性 温度显示一些减数分裂和有丝分裂缺陷，例如染色体不分离、渗透和 机械敏感性和胞质分裂失败。使用各种遗传和细胞生物学技术，我 提议揭示分离酶促进胞质分裂完成的机制 线虫的早期胚胎。通过这个分析，我希望能够扩展对分子的理解。 用于染色体分离与后期开始的时间协调的机制 和胞质分裂。